Open Access
Research (Published online: 08-02-2019)
3. Staphylococcus aureus isolated from selected dairies of Algeria: Prevalence and susceptibility to antibiotics
Asmaa Manel Matallah, Leila Bouayad, Sofiane Boudjellaba, Faiza Mebkhout, Taha Mossadak Hamdi and Nadjia Ramdani-Bouguessa
Veterinary World, 12(2): 205-210

Asmaa Manel Matallah: Laboratory of Food Hygiene and Quality Insurance System (HASAQ), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
Leila Bouayad: Laboratory of Food Hygiene and Quality Insurance System (HASAQ), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
Sofiane Boudjellaba: Research Laboratory Management of Local Animal Resources (GRAAL), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
Faiza Mebkhout: Laboratory of Food Hygiene and Quality Insurance System (HASAQ), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
Taha Mossadak Hamdi: Laboratory of Food Hygiene and Quality Insurance System (HASAQ), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
Nadjia Ramdani-Bouguessa: Laboratory of Medical Biology, Mohamed Mahmoudi City, Number 209 Bis, Baraki, Algiers, Algeria.

doi: 10.14202/vetworld.2019.205-210

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Article history: Received: 19-09-2018, Accepted: 26-12-2018, Published online: 08-02-2019

Corresponding author: Asmaa Manel Matallah


Citation: Matallah AM, Bouayad L, Boudjellaba S, Mebkhout F, Hamdi TM, Ramdani-Bouguessa N (2019) Staphylococcus aureus isolated form selected dairies of Algeria: Prevalence and susceptibility to antibiotics, Veterinary World, 12(2): 205-210.

Aim: The objectives of this study were to assess the prevalence of Staphylococcus aureus in raw milk in Algerian dairies, to study the effect of seasons on the contamination of milk and the susceptibility of isolated strains to antibiotics, and to estimate the risk on the health consumer.

Materials and Methods: The ISO method 6888-1 (1) was used for Staphylococcus screening. Antimicrobial susceptibility to the 11most used antibiotics in veterinary medicine was assessed using the disk diffusion assay.

Results: The overall prevalence was 31.56% (95/301); 34.84% (85/244) from raw milk collectors cisterns (MCC), 22.73% (5/22) from mixing tank milk before pasteurization, and 14.29% (5/35) from pasteurized tank milk (p<0.05). A significant difference (p<0.001) of contamination on MCC was observed between dairies without season influence (p≥0.05). It was observed that 49.47% of S. aureus isolates were resistant to penicillin, 5.26% to tetracycline, 4.21% to erythromycin, 3.15% to neomycin, 2.10% to cefoxitin, 2.10% to clindamycin, and 1.05% to ofloxacin. No resistance was observed for vancomycin, gentamicin, chloramphenicol, and trimethoprim-sulfamethoxazole.

Conclusion: A high prevalence of S. aureus from MCC was observed without significant effect of season. The pasteurization does not ensure the elimination of bacteria in all samples. Half of the isolates were resistant to penicillin. These findings emphasize the importance of S. aureus control in Algerian milk industry at different levels to improve public health.

Keywords: antibiotic susceptibility, pasteurized milk, raw milk, Staphylococcus aureus.


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