Vet World Vol.12 January-2019 Article-19
Research Article
Veterinary World, 12(1): 131-135
https://doi.org/10.14202/vetworld.2019.131-135
Molecular evidence of Ehrlichia canis and Anaplasma platys and the association of infections with hematological responses in naturally infected dogs in Kalasin, Thailand
2. One Health Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand.
3. Department of Infectious Disease Control, Faculty of Medicine, Oita University, Oita, Japan.
Background and Aim: This study aimed to screen A. platys and E. canis in naturally infected dogs and the effects of the infection on the levels of packed cell volume (PCV) and platelet count.
Materials and Methods: A total of 68 blood samples were collected from free-roaming dogs at Nong Kung Sri district, Kalasin Province, Thailand, and examined for A. platys and E. canis infection by polymerase chain reaction (PCR) and measured PCV levels and platelet count.
Results: Using nested PCR, 42.65% of dogs were infected with one or two pathogens. The molecular detection of anaplasmosis and ehrlichiosis in this population was 29.4% (95% confidence interval [CI]: 18.98-41.71) and 25% (95% CI: 14.4-35.3), respectively. Coinfection occurred at 11.8% (95% CI: 5.22-21.87). Infection with E. canis and coinfection showed significant association with PCV levels (p<0.05) while A. platys infection showed no statistical relationship. Infection with A. platys, E. canis, and coinfection had a non-significant correlation with platelet count (p>0.05).
Conclusion: This study provides data of anaplasmosis and ehrlichiosis in free-roaming dogs which indicated that these zoonotic diseases are widespread and require for disease frequency determination, especially in Kalasin Province of Thailand where data of tick-borne infections in dogs have not been reported. Keywords: packed cell volume, platelet count, Thailand, tick-borne pathogens.
Keywords: packed cell volume, platelet count, Thailand, tick-borne pathogens.
How to cite this article: Piratae S, Senawong P, Chalermchat P, Harnarsa W, Sae-Chue B (2019) Molecular evidence of Ehrlichia canis and Anaplasma platys and the association of infections with hematological responses in naturally infected dogs in Kalasin, Thailand, Veterinary World, 12(1): 131-135.
Received: 10-09-2018 Accepted: 12-12-2018 Published online: 23-01-2019
Corresponding author: Supawadee Piratae E-mail: bios.tah@gmail.com
DOI: 10.14202/vetworld.2019.131-135
Copyright: Piratae, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.