Antigen of 49.6-kDa subunit pili protein of Helicobacter pylori as a potential biomarker for early and rapid detection of the infection

Vet World Vol.12 June-2019 Article-6

Research Article

Veterinary World, 12(6): 769-773

https://doi.org/10.14202/vetworld.2019.769-773

Hamong Suharsono¹, Zainul Muttaqin², I Wayan Masa Tenaya³, Kadek Karang Agustina⁴, and Sumarno Retro Prawiro⁵

1. Laboratory of Biochemistry, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.
2. Biomedical Research Unit, West Nusa Tenggara General Hospital, Lombok, Indonesia.
3. Animal Diseases Investigation Center, Denpasar, Indonesia.
4. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.
5. Laboratory of Microbiology, Medical Faculty of Brawijaya University, Malang, Indonesia.

Background and Aim: Helicobacter pylori infection has been identified as a major cause of peptic ulcer diseases, including gastric and duodenal ulcers, gastritis, chronic and gastric carcinoma, and even gastric lymphoma. In vitro studies using Western blotting analysis, hemagglutination test, adherence inhibition assays, and immunocytochemical staining revealed that the 49.6-kDa subunit pili protein of H. pylori was considered an immunogenic protein. This study aimed to develop a serological diagnostic test using 49.6 kDa for detecting antibodies against H. pylori proteins in an early phase of the infection.

Materials and Methods: An in-house immunochromatographic test (ICT) kit was developed and used to test a panel of sera sample obtained from a randomly selected symptomatic patient, in which 40 sera were H. pylori positive and 40 sera were H. pylori negative.

Results: The results showed that ICT with 49.6 kDa as an antigen was highly sensitive and specific for detecting anti-H. pylori immunoglobulin G antibodies in human serum, with a high negative predictive value.

Conclusion: The developed test could be used to exclude H. pylori infection in symptomatic patients. Keywords: 49.6-kDa pili protein, Helicobacter pylori, immunochromatographic test.

Keywords: 49.6-kDa pili protein, Helicobacter pylori, immunochromatographic test.

How to cite this article: Suharsono H, Muttaqin Z, Tenaya IWM, Agustina KK, Prawiro SR (2019) Antigen of 49.6-kDa subunit pili protein of Helicobacter pylori as a potential biomarker for early and rapid detection of H. pylori infection, Veterinary World, 12(6): 769-773.

Received: 16-01-2019 Accepted: 09-04-2019 Published online: 10-06-2019

Corresponding author: Hamong Suharsono E-mail: hamong@unud.ac.id

DOI: 10.14202/vetworld.2019.769-773

Copyright: Suharsono, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.