Vet World Vol.12 May-2019 Article-2
Research Article
Veterinary World, 12(5): 629-637
https://doi.org/10.14202/vetworld.2019.629-637
Screening commercial teat disinfectants against bacteria isolated from bovine milk using disk diffusion
2. Cellular Health and Toxicology Research Group, Institute of Technology Sligo, County Sligo, Ireland.
3. Department of Food Safety, Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.
Background and Aim: Teat disinfection is an important tool in reducing the incidence of bovine mastitis. Identifying the potential mastitis-causing bacterial species in milk can be the first step in choosing the correct teat disinfectant product. The objective of this study was to screen commercial teat disinfectants for inhibition against mastitis-associated bacteria isolated from various types of milk samples.
Materials and Methods: Twelve commercially available teat disinfectant products were tested, against 12 mastitis-associated bacteria strains isolated from bulk tank milk samples and bacterial strains isolated from clinical (n=2) and subclinical (n=3) quarter foremilk samples using the disk diffusion method.
Results: There was a significant variation (7-30 mm) in bacterial inhibition between teat disinfection products, with products containing a lactic acid combination (with chlorhexidine or salicylic acid) resulting in the greatest levels of bacterial inhibition against all tested bacteria (p<0.05).
Conclusion: In this study, combined ingredients in teat disinfection products had greater levels of bacterial inhibition than when the ingredients were used individually. The disk diffusion assay is a suitable screening method to effectively differentiate the bacterial inhibition of different teat disinfectant products. Keywords: bacteria, disk diffusion, mastitis, teat disinfectant.
Keywords: bacteria, disk diffusion, mastitis, teat disinfectant.
How to cite this article: Fitzpatrick SR, Garvey M, Jordan K, Flynn J, O'Brien B, Gleeson D (2019) Screening commercial teat disinfectants against bacteria isolated from bovine milk using disk diffusion, Veterinary World, 12(5): 629-637.
Received: 04-12-2018 Accepted: 13-03-2019 Published online: 06-05-2019
Corresponding author: David Gleeson E-mail: david.gleeson@teagasc.ie
DOI: 10.14202/vetworld.2019.629-637
Copyright: Fitzpatrick, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.