Open Access
Research (Published online: 21-08-2020)
26. Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa)
Jirapat Ninsuwon, Pitchaporn Waiyamitra, Atthaporn Roongsitthichai and Win Surachetpong
Veterinary World, 13(8): 1667-1673

Jirapat Ninsuwon: Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand; Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand.
Pitchaporn Waiyamitra: Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
Atthaporn Roongsitthichai: Veterinary Clinic Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand; Office of Academic Affairs, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand.
Win Surachetpong: Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand; Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand.

doi: www.doi.org/10.14202/vetworld.2020.1667-1673

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Article history: Received: 30-03-2020, Accepted: 30-06-2020, Published online: 21-08-2020

Corresponding author: Atthaporn Roongsitthichai

E-mail: atthaporn.r@msu.ac.th

Citation: Ninsuwon J, Waiyamitra P, Roongsitthichai A, Surachetpong W (2020) Expressions of miR-155 and miR-181 and predictions of their structures and targets in pigs (Sus scrofa), Veterinary World, 13(8): 1667-1673.
Abstract

Background and Aim: MicroRNAs (miRNAs) are responsible for gene expression control at the post-transcription level in many species. Several miRNAs are required in the regulation of immune responses, such as B-cell differentiation, T-cell receptor signaling pathway, CD4+ T cell selection, and so on. Studies on miRNAs have been extensively conducted in humans and mice; however, reports relevant to miRNAs, especially miR-155 and miR-181, in pigs are limited. Consequently, the present study aimed to investigate the structures, target genes, and expressions of miR-155 and miR-181 in various porcine cells and tissues.

Materials and Methods: Five healthy male pigs from a porcine reproductive and respiratory syndrome virus-negative farm were studied. Before slaughter, blood samples were collected for peripheral blood mononuclear cell isolation. After slaughter, samples of spleen, lymph nodes, and forelimb muscles were collected. Both miR-155 and miR-181 were investigated for their structures with RNAfold web server, for their target genes from three online web servers, and for their expressions using polymerase chain reaction (PCR).

Results: The structures of miR-155 and miR-181 contained hairpins with free energies of –35.27 and –35.29 kcal/mole, respectively. Target gene prediction revealed that miR-155 had perfect complementarity with Socs1 and Mapk3k14, while miR-181 had perfect complementarity with Ddx3x, Nfat5, Foxp1, and Mpp5. PCR showed that both miRNAs were detectable from all investigated cells and tissues. Moreover, the highest expression of both miRNAs was found from the lymph node of the pigs.

Conclusion: Both miR-155 and miR-181 might be involved with the regulation of porcine immune functions as both miRNAs were detected in several cells and tissues of the pigs. In addition, they had very high complementarities with the seed regions of several immune-related genes.

Keywords: immune system, microRNA, miR-155, miR-181, porcine.