Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms

Vet World Vol.13 September-2020 Article-8

Research Article

Veterinary World, 13(9): 1798-1806

https://doi.org/10.14202/vetworld.2020.1798-1806

Supaphorn Seetaha^1,2, Siriluk Ratanabunyong^2,3, Lueacha Tabtimmai², Kiattawee Choowongkomon², Jatuporn Rattanasrisomporn^1,4, and Khuanjarat Choengpanya⁵

1. Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand.
2. Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
3. Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
4. Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
5. Program in Applied Biology, Maejo University Phrae Campus, Phrae 54140, Thailand.

Background and Aim: Feline immunodeficiency virus (FIV) causes AIDS-like symptoms in domestic and wild cats. Treatment of infected cats has been performed using human anti-HIV drugs, which showed some limitations. This study aimed to determine the anti-FIV potential of some mushrooms.

Materials and Methods: A total of 17 medicinal and edible mushrooms were screened to find their inhibitory effect against FIV reverse transcriptase (FIV-RT). Three solvents, water, ethanol, and hexane, were used to prepare crude mushroom extracts. Fluorescence spectroscopy was used to perform relative inhibition and 50% inhibitory concentrations (IC50) studies.

Results: The ethanol extract from dried fruiting bodies of Inonotus obliquus showed the strongest inhibition with an IC50 value of 0.80±0.16 μg/mL. The hexane extract from dried mycelium of I. obliquus and ethanol and water extracts from fresh fruit bodies of Phellinus igniarius also exhibited strong activities with the IC50 values of 1.22±0.20, 4.33±0.39, and 6.24±1.42 μg/mL, respectively. The ethanol extract from fresh fruiting bodies of Cordyceps sinensis, hexane extracts from dried mycelium of I. obliquus, ethanol extracts of Ganoderma lucidum, hexane extracts of fresh fruiting bodies of Morchella esculenta, and fresh fruiting bodies of C. sinensis showed moderate anti-FIV-RT activities with IC50 values of 29.73±12.39, 49.97±11.86, 65.37±14.14, 77.59±8.31, and 81.41±17.10 μg/mL, respectively. These mushroom extracts show anti-FIV potential.

Conclusion: The extracts from I. obliquus, P. igniarius, C. sinensis, and M. esculenta showed potential anti-FIV activity. Keywords: feline immunodeficiency virus, fluorescence spectroscopy, mushrooms, crude extracts, reverse transcriptase.

Keywords: feline immunodeficiency virus, fluorescence spectroscopy, mushrooms, crude extracts, reverse transcriptase.

How to cite this article: Seetaha S, Ratanabunyong S, Tabtimmai L, Choowongkomon K, Rattanasrisomporn J, Choengpanya K (2020) Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms, Veterinary World, 13(9): 1798-1806.

Received: 19-03-2020 Accepted: 06-07-2020 Published online: 05-09-2020

Corresponding author: Jatuporn Rattanasrisomporn E-mail: fvetjpn@ku.ac.th

DOI: 10.14202/vetworld.2020.1798-1806

Copyright: Seetaha, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.