Open Access
Research (Published online: 11-08-2021)
12. Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility
Suherni Susilowati, Imam Mustofa, Wurlina Wurlina, Indah Norma Triana, Suzanita Utama and Rimayanti Rimayanti
Veterinary World, 14(8): 2073-2084

Suherni Susilowati: Laboratory of Veterinary Artificial Insemination, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Mulyorejo, Surabaya, Indonesia.
Imam Mustofa: Laboratory of Veterinary Obstetrics, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Mulyorejo, Surabaya, Indonesia.
Wurlina Wurlina: Laboratory of Veterinary Infertility and Sterility, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Mulyorejo, Surabaya, Indonesia.
Indah Norma Triana: Laboratory of Veterinary Infertility and Sterility, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Mulyorejo, Surabaya, Indonesia.
Suzanita Utama: Laboratory of Veterinary Obstetrics, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Mulyorejo, Surabaya, Indonesia.
Rimayanti Rimayanti: Laboratory of Veterinary Infertility and Sterility, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Unair, Mulyorejo, Surabaya, Indonesia.

doi: www.doi.org/10.14202/vetworld.2021.2073-2084

Share this article on [Facebook] [LinkedIn]

Article history: Received: 26-03-2021, Accepted: 01-07-2021, Published online: 11-08-2021

Corresponding author: Imam Mustofa

E-mail: imam.mustofa@fkh.unair.ac.id

Citation: Susilowati S, Mustofa I, Wurlina W, Triana IN, Utama S, Rimayanti R (2021) Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility, Veterinary World, 14(8): 2073-2084.
Abstract

Background and Aim: Kacang buck sperm is cryosensitive due to the seminal plasma of semen itself. Meanwhile, bull seminal plasma contains the insulin-like growth factor-1 (IGF-1) complex, which is cryoprotective. The addition of the crude protein of Simmental bull seminal plasma increased the quality of post-thawed semen of Kacang buck. The study was conducted to determine the effects of Simmental bull seminal plasma with IGF-1 on the fertility of post-thawed Kacang buck semen.

Materials and Methods: Buck semen was diluted in the following skim milk-egg yolk extender preparations: Without the addition of Simmental bull seminal plasma IGF-1 complex protein (T0); with the addition of 12-μg Simmental bull seminal plasma IGF-1 complex protein (T1); and with the addition of 24-μg Simmental bull seminal plasma IGF-1 complex protein (T2). The extended semen was packed in 0.25-mL straws and frozen. Post-thawed semen fertility was evaluated based on the following variables: Sperm motility, viability, intact plasma membrane (IPM), malondialdehyde (MDA) levels, capacitation status, and acrosome reaction. The difference in each variable among the groups was evaluated using analysis of variance, followed by Tukey's honestly significant difference test, at a 95% level of significance. Meanwhile, principal component analysis (PCA) was used to identify the principal component of semen fertility among the seven parameters.

Results: The T1 group showed the highest sperm motility, viability, IPM, and percentage of incapacitated sperm and the lowest MDA levels, percentage of capacitated sperm, and acrosome reaction. PCA revealed that sperm motility had a moderate to very robust correlation with other variables and is the most crucial parameter, accounting for 80.79% of all variables.

Conclusion: The IGF-1 complex in Simmental bull seminal plasma was useful for increasing the fertility of post-thawed Kacang buck semen, and sperm motility was the principal component of semen fertility.

Keywords: acrosome reaction, capacitation, good health and well-being, intact plasma membrane, motility, viability.