Dynamic change of blood profile in rat models with acute skin injury artificially infected with methicillin-resistant Staphylococcus aureus

Vet World Vol.14 August-2021 Article-13

Research Article

Veterinary World, 14(8): 2085-2090

https://doi.org/10.14202/vetworld.2021.2085-2090

Yos Adi Prakoso¹, Nurul Hidayah¹, Chylen Setiyo Rini², and Kurniasih Kurniasih³

1. Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, East Java, 60225, Indonesia.
2. Integrated Laboratory, Faculty of Health Sciences, University of Muhammadiyah Sidoarjo, East Java 61262, Indonesia.
3. Department of Pathology, Faculty of Veterinary Medicine, University of Gadjah Mada, Yogyakarta 55281, Indonesia.

Background and Aim: A wound is a common problem for humans and animals. The wound becomes more severe if it is infected by bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA). The wound healing mechanism involves various factors, either in the local tissue or the bloodstream. However, the presentation of infected wound healing regarding its impacts on the dynamic change of blood profile is not clearly understood. This study aimed to explore the impacts of wound creation on the blood profile in rat models with and without being artificially infected by MRSA.

Materials and Methods: Thirty male Sprague-Dawley rats (6 months old; weight, 300 g) were used as the model. They were divided into three groups: Without wound creation (C), wounded without infection (CW), and wounded and artificially infected by MRSA (CWI). Groups CW and CWI were shaved and induced with 4 mm two-round full-thickness biopsy on the back. Furthermore, group CWI was artificially infected by 105 colony-forming units of MRSA. The blood samples were collected through the tail vein from days 1 to 5. The blood parameters included blood profile, total plasma protein, C-reactive protein, CD4+, CD8+, CD4+/CD8+, and COX-2. The data were analyzed using the Statistical Package for the Social Sciences, version 16 (SPSS, IBM, Armonk, NY, USA).

Results: The result showed that the presentation of a wound with and without MRSA infection significantly changed the total erythrocytes, leukocytes, neutrophils, lymphocytes, total plasma protein, C-reactive protein, and the subset of circulatory CD4+, CD8+, and COX-2 (p≤0.05). In addition, the wound infected with MRSA impacts the mean corpuscular volume (p≤0.05).

Conclusion: Moreover, the presentation of the wound with and without MRSA infection induces dynamic changes on various blood profile parameters. Keywords: blood profile, infection, methicillin-resistant Staphylococcus aureus, protein profile, wound.

Keywords: blood profile, infection, methicillin-resistant Staphylococcus aureus, protein profile, wound.

How to cite this article: Prakoso YA, Hidayah N, Rini CS, Kurniasih K (2021) Dynamic change of blood profile in rat models with acute skin injury artificially infected with methicillin-resistant Staphylococcus aureus, Veterinary World, 14(8): 2085-2090.

Received: 06-03-2021 Accepted: 09-07-2021 Published online: 11-08-2021

Corresponding author: Kurniasih Kurniasih E-mail: kurniasih_1951@yahoo.co.id

DOI: 10.14202/vetworld.2021.2085-2090

Copyright: Prakoso, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.