Vet World Vol.14 August-2021 Article-25
Research Article
Veterinary World, 14(8): 2170-2177
https://doi.org/10.14202/vetworld.2021.2170-2177
Comparative study of the therapeutic efficacy of autologous platelet-rich plasma and honey in healing skin wounds in sheep
2. Biotechnology's Laboratory of the Bioactive Molecules and the Cellular Physiopathology, University of Batna 2, Batna, Algeria.
3. Laboratory of Biology and Environment, Faculty of Nature and Life Sciences, University of Mentouri Brothers, Constantine, Algeria.
Background and Aim: This investigation is the continuation of a published preliminary study examining the therapeutic efficacy of platelet-rich plasma (PRP) as a topical treatment for skin wounds in sheep. The study aimed to compare the healing effects of autologous PRP with that of natural honey.
Materials and Methods: This study involved nine clinically healthy male sheep. After sterile skin preparation, full-thickness longitudinal incision wounds were created on the backs of each animal. The animals were randomly divided into three groups of three sheep each. In Group I, the wounds were treated with PRP; in Group II, the wounds were treated with honey; and in Group III, the wounds were treated with saline solution. The different treatments were administered topically every 3 days. Healing was assessed by a semi-quantitative histopathological study from biopsies taken on the 3rd, 7th, 14th, 21st, and 28th days of healing. The data obtained were compared using the non-parametric Mann–Whitney U-test, and p<0.05 and 0.01 were used to determine the level of significance of the recorded differences.
Results: Semi-quantitative histopathological evaluation showed significant differences in the progression of wound healing between the three study groups. Recorded data showed that PRP may reduce inflammation during the first 3 days after the incision. Moreover, the synthesis and organization of collagen fibers were significantly improved in the group treated with PRP compared with those in the group treated with honey.
Conclusion: PRP offers a promising therapeutic option for healing skin wounds in sheep compared with honey. Keywords: honey, platelet-rich plasma, sheep, skin, wound healing.
Keywords: honey, platelet-rich plasma, sheep, skin, wound healing.
How to cite this article: Badis D, Ouafa D (2021) Comparative study of the therapeutic efficacy of autologous platelet-rich plasma and honey in healing skin wounds in sheep, Veterinary World, 14(8): 2170-2177.
Received: 07-04-2021 Accepted: 16-07-2021 Published online: 23-08-2021
Corresponding author: Daikh Badis E-mail: badis.daikh@univ-batna2.dz
DOI: 10.14202/vetworld.2021.2170-2177
Copyright: Badis and Ouafa, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.