Evaluation of sodium lauryl sulfate for the development of cow-side mastitis screening test

Vet World Vol.14 August-2021 Article-40

Research Article

Veterinary World, 14(8): 2290-2295

https://doi.org/10.14202/vetworld.2021.2290-2295

Nobonita Sarker Tanni¹, Md. Shafiul Islam^1,2, Mojahidul Kabir^1,3, Mst. Sonia Parvin¹, Md. Amimul Ehsan¹, and Md. Taohidul Islam¹

1. Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
2. Poultry and Livestock Program (Ultra Poor Graduation Program), BRAC, Barishal, Bangladesh.
3. Department of Livestock Services, Farmgate, Dhaka, Bangladesh.

Background and Aim: Subclinical mastitis (SCM) is an economically important disease for dairy cattle worldwide; therefore, regular screening is imperative to detect SCM at an early stage so as to control it. The study was conducted to compare the test characteristics of sodium lauryl sulfate (SLS) as a test reagent to detect SCM in dairy cows.

Materials and Methods: First, 106 milk samples of dairy cows were subjected to available indirect screening tests (white side test [WST], surf field mastitis test, Leucocytest, and Immucell) considering somatic cell count (SCC) as gold standard test. Then 273 milk samples were allowed to react with different concentrations of SLS with or without sodium hydroxide (NaOH) and indicators (bromothymol blue [BTB] and bromocresol purple [BCP]).

Results: SLS with or without NaOH yielded best reaction with the milk samples similar to Leucocytest. It was observed that the reaction of milk samples with SLS added with indicators (BTB and BCP) was easier to visualize than without indicators. SLS 3%+NaOH 1.5% with BTB and SLS 2% with BCP had high sensitivity, specificity, and substantial agreement with SCC. The area under the receiver operating characteristics curve of SLS 2% with BCP and SLS 3%+NaOH 1.5% with BTB was 0.917 and 0.875, respectively.

Conclusion: It may be concluded that SLS 3%+NaOH 1.5% with BTB and SLS 2% with BCP may be the potential reagents for the development of an effective cow-side test to detect SCM, as the main ingredient SLS is considerably cheap and readily available in developing countries. Keywords: bovine subclinical mastitis, dairy cows, screening test, sensitivity, sodium lauryl sulfate, specificity.

Keywords: bovine subclinical mastitis, dairy cows, screening test, sensitivity, sodium lauryl sulfate, specificity.

How to cite this article: Tanni NS, Islam MS, Kabir M, Parvin MS, Ehsan MA, Islam MT (2021) Evaluation of sodium lauryl sulfate for the development of cow-side mastitis screening test, Veterinary World, 14(8): 2290-2295.

Received: 09-04-2021 Accepted: 29-07-2021 Published online: 31-08-2021

Corresponding author: Md. Taohidul Islam E-mail: taohid@bau.edu.bd

DOI: 10.14202/vetworld.2021.2290-2295

Copyright: Tanni, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.