Anticancer activity of milk fat rich in conjugated linoleic acid against Ehrlich ascites carcinoma cells in female Swiss albino mice

Vet World Vol.14 March-2021 Article-20

Research Article

Veterinary World, 14(3): 696-708

https://doi.org/10.14202/vetworld.2021.696-708

Abdelrahman M. Abd El-Gawad¹, Diea G. Abo El-Hassan², Ahmed M. Aboul-Enein³, Sherein S. Abdelgayed⁴, Salwa A. Aly⁵, Gamal Esmat⁶, Amr A. Mostafa³, Mohamed H. Bakr¹, Rida A. Ali¹, and Mahmoud A. Ayoub⁷

1. Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt.
2. Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
3. Department of Biochemistry, Faculty of Agriculture, Cairo University, Giza, Egypt.
4. Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
5. Department of Food Hygiene, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
6. Department of Hepatogastroenterology and Infectious Diseases, Faculty of Medicine, Cairo University, Giza, Egypt.
7. Department of Clinical and Chemical Pathology, National Cancer Institute, Cairo University, Giza, Egypt.

Background and Aim: The major conjugated linoleic acid (CLA) isomers have anticancer effect, especially breast cancer cells, inhibits cell growth and induces cell death. Also, CLA has several health benefits in vivo, including antiatherogenesis, antiobesity, and modulation of immune function. The present study aimed to assess the safety and anticancer effects of milk fat CLA against in vivo Ehrlich ascites carcinoma (EAC) in female Swiss albino mice. This was based on acute toxicity study, detection of the tumor growth, life span of EAC bearing hosts, and simultaneous alterations in the hematological, biochemical, and histopathological profiles.

Materials and Methods: One hundred and fifty adult female mice were equally divided into five groups. Groups (1-2) were normal controls, and Groups (3-5) were tumor transplanted mice (TTM) inoculated intraperitoneally with EAC cells (2×106/0.2 mL). Group (3) was (TTM positive control). Group (4) TTM fed orally on balanced diet supplemented with milk fat CLA (40 mg CLA/kg body weight). Group (5) TTM fed orally on balanced diet supplemented with the same level of CLA 28 days before tumor cells inoculation. Blood samples and specimens from liver and kidney were collected from each group. The effect of milk fat CLA on the growth of tumor, life span of TTM, and simultaneous alterations in the hematological, biochemical, and histopathological profiles were examined.

Results: For CLA treated TTM, significant decrease in tumor weight, ascetic volume, viable Ehrlich cells accompanied with increase in life span were observed. Hematological and biochemical profiles reverted to more or less normal levels and histopathology showed minimal effects.

Conclusion: The present study proved the safety and anticancer efficiency of milk fat CLA and provides a scientific basis for its medicinal use as anticancer attributable to the additive or synergistic effects of its isomers. Keywords: anticancer activity, conjugated linoleic acid, Ehrlich ascites carcinoma, % increase in life span, mean survival time, tumor transplanted mice.

Keywords: anticancer activity, conjugated linoleic acid, Ehrlich ascites carcinoma, % increase in life span, mean survival time, tumor transplanted mice.

How to cite this article: Abd El-Gawad AM, Abo El-Hassan DG, Aboul-Enein AM, Abdelgayed SS, Aly SA, Esmat G, Mostafa AA, Bakr MH, Ali RA, Ayoub MA (2021) Anticancer activity of milk fat rich in conjugated linoleic acid against Ehrlich ascites carcinoma cells in female Swiss albino mice, Veterinary World, 14(3): 696-708.

Received: 21-09-2020 Accepted: 02-02-2021 Published online: 20-03-2021

Corresponding author: Diea G. Abo El-Hassan E-mail: dieaabo@cu.edu.eg

DOI: 10.14202/vetworld.2021.696-708

Copyright: Abd El-Gawad, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.