Vet World Vol.14 October-2021 Article-17
Research Article
Veterinary World, 14(10): 2699-2704
https://doi.org/10.14202/vetworld.2021.2699-2704
Wound healing by topical application of Momordica charantia L. formulations on mice
2. Department of Biochemistry, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Peru.
3. Graduate School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Peru.
4. Department of Biological Chemistry and Animal Physiology, School of Biological Science, National University of Trujillo, Trujillo, Peru.
5. Department of Physiology, School of Medicine, National University of Trujillo, Trujillo, Peru.
Background and Aim: Momordica charantia is mainly characterized by its antimicrobial and antioxidant properties. The current study aimed to evaluate the healing activity of gel and cream formulations based on M. charantia on induced wounds in mice.
Materials and Methods: Acetonic extract of M. charantia was prepared and incorporated into gel and cream formulations. Mus musculus Balb/c (n=30) with induced injury were distributed into five groups: Group I (control – day 7), Group II (control – day 14), Group III (1% gel – day 7), and Group IV (1% gel – day 14) to which 1% M. charantia gel was dermally applied daily for 7 and 14 days, respectively, Group V (1% cream – day 7) and Group VI (1% cream – day 14) to which of M. charantia 1% cream were dermally applied daily for 7 and 14 days, respectively. Time of wound closure was determined during the experimentation; rats were euthanized with sodium pentobarbital 60 mg/kg/pc v.ip. for obtaining skin samples for histopathological analysis.
Results: Groups IV and VI showed a higher percentage of wound closure on day 14, and in histopathological analysis, effect was greater in Group VI with the presence of fibroblasts and abundant collagen and elastic fibers.
Conclusion: M. charantia gel and cream showed wound healing activity on induced wounded mice; the most effective treatment was M. charantia 1% cream formulation. Keywords: histology, Momordica charantia, skin, topical administration, wound healing.
Keywords: histology, Momordica charantia, skin, topical administration, wound healing.
How to cite this article: Sagástegui-Guarniz WA, Silva-Correa CR, Villarreal-La Torre VE, González-Blas MV, Sagástegui-Guarniz WO, Calderón-Peña AA, Aspajo-Villalaz CL, Cruzado-Razco JL, Hilario-Vargas J (2021) Wound healing by topical application of Momordica charantia L. formulations on mice. Veterinary World, 14(10): 2699-2704.
Received: 27-04-2021 Accepted: 09-09-2021 Published online: 22-10-2021
Corresponding author: William Antonio Sagástegui-Guarniz E-mail: wsagastegui@unitru.edu.pe
DOI: 10.14202/vetworld.2021.2699-2704
Copyright: Sagástegui-Guarniz, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.