Open Access
Research (Published online: 13-04-2022)
13. The effect of bile salt diet supplementation on genes related to fat metabolism in yellow-feathered broilers
Zhenming Zhang, Baoan Ding, Hailian He, Jingge Wang, Xiongjie Liu, Jiahui Guo, Pengxiang Li and Stephen R. Madigosky
Veterinary World, 15(4): 911-918

Zhenming Zhang: Department of Animal Science, College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China.
Baoan Ding: Department of Animal Science, College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China.
Hailian He: Department of Animal Science, College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China.
Jingge Wang: Department of Animal Science, College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China.
Xiongjie Liu: Department of Animal Science, College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China.
Jiahui Guo: Department of Animal Science, College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China.
Pengxiang Li: Department of Animal Science, College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China.
Stephen R. Madigosky: Department of Environmental Science and Biology, One University Place, Widener University, Chester, Pennsylvania 19013, USA.

doi: www.doi.org/10.14202/vetworld.2022.911-918

Share this article on [Facebook] [LinkedIn]

Article history: Received: 10-12-2021, Accepted: 01-03-2022, Published online: 13-04-2022

Corresponding author: Baoan Ding

E-mail: dingbaoan1967@aliyun.com

Citation: Zhang Z, Ding B, He H, Wang J, Liu X, Guo J, Li P, Madigosky SR (2022) The effect of bile salt diet supplementation on genes related to fat metabolism in yellow-feathered broilers, Veterinary World, 15(4): 911-918.
Abstract

Background and Aim: As a new feed additive, bile acid (BA) can promote the absorption and transport of lipids and fat-soluble vitamins. In recent years, BAs have been widely used in animal feed to promote fat absorption. Therefore, this study aimed to investigate the effect of bile salt supplementation in the diet of yellow-feathered broilers on messenger RNA (mRNA) expression of sterol regulatory element-binding protein 1 (SREBF1), fatty acid synthase (FAS), acetyl-coenzyme A carboxylase (ACC), and fatty acid transport protein 4 (FATP4).

Materials and Methods: Four hundred and twenty commercial male chicks were randomly divided into seven groups (with four replicates per group and 15 chickens per replicate). They were fed diets supplemented with bile salts at 0, 1.5, 2.5, 3.5, 4.5, 5.5 mg/kg, and 2 mg/kg tylosin for 30 days. Changes in SREBF1, fatty acid transporter 4, FAS, and acetyl- CoA carboxylase genes in intestinal mucosa and liver of yellow-feathered broilers were determined using a quantitative fluorescence polymerase chain reaction.

Results: mRNA expression of SREBF1, FAS, ACC, and FATP4 in the small intestine decreased in chicks fed diets supplemented with 3.5 and 4.5 mg/kg bile salts (p<0.05) compared with the control group on 7 days and 14 d. The mRNA expressions of SREBF1, FAS, ACC, and FATP4 in liver tissue decreased in chicks fed diets supplemented with 4.5 and 5.5 mg/kg bile salts (p<0.05) compared to the control group on 7 days. The mRNA expression of SREBF1, FAS, ACC, and FATP4 in the liver at 14 days and the small intestine on 21 days also decreased in chicks fed diets supplemented with 4.5 mg/kg bile salts (p<0.05) compared to the control group. When contrasted with the control group on day 21, the mRNA expression of SRWBF1, FAS, ACC, and FATP4 detected in the liver was lower in chicks fed diets supplemented with bile salts (p<0.05).

Conclusion: The dietary supplementation of bile salts at 4.5 mg/kg effectively regulates the expression of fat metabolism genes, such as SREBF1, FAS, ACC, and FATP4 mRNA. At this concentration, bile salts promote fat catabolism, inhibit fat synthesis, and play an essential role in improving the fat deposition of broilers.

Keywords: acetyl-CoA carboxylase, fat metabolism, fatty acid synthase, fatty acid transporter 4, sterol regulatory element-binding transcription factor 1, supplemental dietary bile salts.