Open Access
Research (Published online: 23-07-2022)
21. Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia
Faisal Fikri, Dhandy Koesoemo Wardhana, Agus Purnomo, Shafia Khairani, Shekhar Chhetri and Muhammad Thohawi Elziyad Purnama
Veterinary World, 15(7): 1759-1764

Faisal Fikri: Department of Veterinary Science, Division of Veterinary Clinical Pathology and Physiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, Indonesia.
Dhandy Koesoemo Wardhana: Department of Veterinary Science, Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Agus Purnomo: Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Shafia Khairani: Department of Biomedical Science, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia.
Shekhar Chhetri: Department of Animal Science, College of Natural Resources, Royal University of Bhutan, Lobesa, Punakha, Bhutan.
Muhammad Thohawi Elziyad Purnama: Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, Indonesia; Department of Veterinary Science, Division of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.

doi: www.doi.org/10.14202/vetworld.2022.1759-1764

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Article history: Received: 08-03-2022, Accepted: 31-05-2022, Published online: 23-07-2022

Corresponding author: Muhammad Thohawi Elziyad Purnama

E-mail: thohawi@fkh.unair.ac.id

Citation: Fikri F, Wardhana DK, Purnomo A, Khairani S, Chhetri S, Purnama MTE (2022) Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia, Veterinary World, 15(7): 1759–1764.
Abstract

Background and Aim: Motile Aeromonas septicemia is a crucial disease in freshwater fish. Aeromonas hydrophila is a disease agent associated with sporadic fish mortality, food safety, and public health. This study aimed to estimate the prevalence and the presence of the aerolysin gene and antimicrobial resistance profile of A. hydrophila isolated from milkfish in Gresik, Indonesia.

Materials and Methods: A total of 153 milkfish gill samples were collected from 16 locations in Gresik and then cultured and identified using biochemical tests. The aerolysin gene was investigated using a polymerase chain reaction, and antimicrobial resistance profiles of the recovered isolates were investigated.

Results: Of the 153 examined samples, 35 (22.9%) were confirmed positive for A. hydrophila and 22 (62.9%) presented the aerolysin gene. The recovered isolates were resistant to the following antibiotics: Amoxicillin (62.9%), tetracycline (60%), streptomycin (54.3%), cefotaxime (51.4%), gentamycin (31.4%), kanamycin (28.6%), erythromycin (25.7%), chloramphenicol (20%), and trimethoprim (14.3%). Meanwhile, only ciprofloxacin, nalidixic acid, and imipenem were indicated as susceptible.

Conclusion: The presence of the aerolysin gene is vital in determining the virulence of A. hydrophila. The study results indicated a high aerolysin gene prevalence. In addition, this study emphasized antibiotic use monitoring, food safety improvement, and negative impact reduction on human health and the environment.

Keywords: aerolysin gene, Aeromonas hydrophila, antimicrobial resistance, milkfish, public health.