Comparative staining of Rhinolophus spp. white blood cells in blood smears

Vet World Vol.15 March-2022 Article-28

Research Article

Veterinary World, 15(3): 750-756

https://doi.org/10.14202/vetworld.2022.750-756

Astghik Ghazaryan¹, Seda Adamyan¹, Tigran Hayrapetyan¹, George Papov¹, Lina Hakobyan², Liana Abroyan², Nane Bayramyan², Sona Hakobyan², Arpine Poghosyan², Hrag Torossian³, and Zaven Karalyan^2,3

1. Department of Zoology, Faculty of Biology, Yerevan State University, A. Manoogian 1, 0025, Yerevan, Armenia.
2. Laboratory of Cell Biology and Virology, Institute of Molecular Biology NAS RA, Yerevan, Armenia.
3. Department of Medical Biology, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.

Background and Aim: A drawback of studies on bat blood smears in the field is the lack of time for fixation because blood sampling using a non-lethal method often provides less time for fixation in smear preparations due to the small volume of blood collected. Usually, there is insufficient blood for another smear preparation, so it is necessary to use blood smears as rationally as possible, especially for rare bats. Many stains are used for staining peripheral blood smears, and they have advantages and disadvantages. This study aimed to examine commonly used stains for blood smears to select the best stain for staining peripheral blood smears in bats.

Materials and Methods: In this study, 48 blood smears of Rhinolophus spp. bats were examined using several blood staining methods. Four methods that showed the best results were used in further experiments: Romanowsky-Giemsa, Pappenheim, hematoxylin-eosin, and eosin methylene blue.

Results: Comparative analysis of different methods for staining bat blood smears revealed that the most convenient method for analyzing blood cells is Pappenheim method.

Conclusion: Staining blood smears using Pappenheim method yield the least number of unsuccessful blood smear stains and are quite effective for the morphological analysis of blood cells. Keywords: blood smears, Rhinolophidae, staining, white blood cells.

Keywords: blood smears, Rhinolophidae, staining, white blood cells.

How to cite this article: Ghazaryan A, Adamyan S, Hayrapetyan T, Papov G, Hakobyan L, Abroyan L, Bayramyan N, Hakobyan S, Poghosyan A, Torossian H, Karalyan Z (2022) Comparative staining of Rhinolophus spp. white blood cells in blood smears, Veterinary World, 15(3): 750-756.

Received: 18-11-2021 Accepted: 15-02-2022 Published online: 29-03-2022

Corresponding author: Zaven Karalyan E-mail: zkaralyan@yahoo.com

DOI: 10.14202/vetworld.2022.750-756

Copyright: Ghazaryan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.