Open Access
Review (Published online: 21-02-2023)
14. A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science
Barbara Ribeiro de Souza Cortez and Roberto Maurício Carvalho Guedes
Veterinary World, 16(2): 347-356

Barbara Ribeiro de Souza Cortez: Department of Veterinary Clinic and Surgery, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Canada.
Roberto Maurício Carvalho Guedes: Department of Veterinary Clinic and Surgery, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

doi: www.doi.org/10.14202/vetworld.2023.347-356

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Article history: Received: 01-09-2022, Accepted: 06-01-2023, Published online: 21-02-2023

Corresponding author: Roberto Maurício Carvalho Guedes

E-mail: guedes@vet.ufmg.br

Citation: Cortez BRS and Guedes RMC (2023) A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science, Veterinary World, 16(2): 347–356.
Abstract

Different techniques have been reported in studies of intestinal in vitro organ culture (IVOC). A robust compilation of all available methods is lacking in the literature, making it difficult to choose a method that corresponds to the study's demands. In this review, readers can assess the most available methods, allowing them to evaluate which is more suitable for their purposes and requirements. A simplified view of culturing intestinal explants is presented, highlighting the approachability of IVOC. Relevant findings from diverse veterinarian studies, where explants played a major role, as well as the technique used in each, are described to illustrate its applications. Finally, the strengths and limitations of the innovative intestinal IVOC methods are discussed. This review provides a collection of methods for intestinal explant culture and their possible applications in veterinary research. In this way, it aims to broaden access to IVOC techniques and aid decision-making regarding the best suited for a study's purposes.

Keywords: enteropathogens, explants culture, intestinal pathogens, swine colon, ussing chamber.