Veterinary World


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1.  Survival and infectivity studies of in-vitro cultivated larvae of Haemonchus contortus in sheep and goats in Nigeria - A O Sonibare, H A Kumshe, E A Okewole, A 0 Talabi, O. O. Alaka, E B Otesile, R A Joshua
Vet World. 2011; 4(12): 533-536


doi: 10.5455/vetworld.2011.533-536

The survival and infectivity of indigenous isolates of Haemonchus contortus and in vitro cultivated infective larvae (L3) were studied. Active motile gravid females, adult and immature parasites were morphologically and morphometrically characterized and isolated from Haemonchus infected goats. These were subsequently inoculated into the abomasum of parasite free lambs through left sided laparatomy for production of fertile eggs to be used for coproculture. Harvested faeces from these lambs were cultured in a nutritive medium and kept in the incubator at 320C and room temperature 24-280C to obtain in vitro cultivated L3 of H. contortus. The infectivity and survival of in vitro cultivated L3 stored in refrigerator at 4-80C and under room temperature 24-280C were evaluated. Incubation period of coproculture was observed to be shorter in medium at 320C than under room temperature 24-280C. The storage of in vitro cultivated L3 under different temperature range showed that the quantity of active larvae in a given volume decreased at p<0.05 from the 5th day under 24-280C while at 4-80C became significantly lower from 20th day. Infectivity of isolate kept at 4-80C was sustained for 18-20 days while at 24-280C it was maintained for 10-12 days. Laboratory in-vitro cultivated L3 of H. contortus had limited infectivity and larvae mortality increase with time under different temperatures. Keywords: Indigenous goats, Haemonchus contortus, in vitro cultivated L3, storage, survival and infectivity