Effect of Antistress agents on Haemato-Biochemical profiles of broiler and breeder hen druing summer

An experiment was carried out to evaluate the effect of five commonly used antistress agents on haemato-biochemical profiles of broiler and broiler breeder hen. In case of broiler, significant differences were observed between treatment groups for haemoglobin concentration, total RBC and total WBC count, total blood glucose, total serum protein, total serum albumin, total alkaline phosphatase activity and total serum cholesterol level. No definite trend was observed amongst the treatment groups and control for total blood glucose content and total alkaline phosphatase activity. It is apparent that the groups fed with antistress agents like Stress roak and Venlyte had lowest serum cholesterol level compared to other groups. In case of broiler breeder hen difference between treatment groups were significant for haemoglobin content, but not for total RBC and WBC count. Differences between treatment groups although were not significant statistically for total serum protein, however differences between treatment groups were highly significant for total blood glucose.


Introduction
540 day old unsexed chicks of broiler dam line were randomly divided into six groups of 90 chicks.High ambient temperature and humidity are Each 90 chicks forming each experimental group for considered as the major stressors to which poultry are testing of five antistress agents and one group to be exposed in coastal belt of Orissa during summer.used as control.Each group was further sub-divided Elevated heat and humidity are much more stressful to into three sub groups of 30 chicks each to serve as the birds than high temperature alone.This heat stress replicate, thus six treatment groups each having three effect is more pronounced in fast growing commercial replicates.broilers and non selected meat type lines.Considering In addition to this, 216 broiler breeder hens were the above situation, different antistress agents are divided into six groups of 42 birds (36 female and 6 being administered in the diet of broiler and broiler male).The average age of the birds was about 56 breeder hen to overcome heat stress.This article weeks at the beginning of the experiment.Male birds reports the effect of five commonly used antistress were kept for yielding of semen.The experiment agents on haemato-biochemical profiles of broiler and commenced on 1st May and terminated on 30th June.broiler breeder hen.
Five antistress agents were tested separately in

Material and Methods
broiler and broiler breeder hen.In both the cases group-1 was used as control and designated as T1.Diet An experiment was conducted on 216 broiler for group-2 supplemented with ascorbic acid @ 0.8g breeder hens and 540 day old broiler chicks of dam /kg of feed was designated as T2.The diet of the group line maintained at experimental poultry farm, Orissa which was designated as T3 was supplemented with Veterinary College, O.U.A.T., Bhubaneswar.Five sodium bi carbonate @ 15g/kg of feed.The fourth antistress agents were tested.These were Vitamin-C group which was designated as T4 were given Zeetress (ascorbic acid), Sodium bi carbonate, Zeetress ( M/S (M/S Indian Herbs, Saharanpur, U.P.) in drinking Indian Herbs, Saharanpur, U.P.), Stress roak ( M/S water @ 1g/ 100 birds per day.Stress roak ( M/S Dabur Dabur India Pvt. Ltd., New Delhi) and Venlyte (M/S India Pvt. Ltd., New Delhi ) @ 10 ml / 100 birds per Venky Pharmaceutical of Venkateswar Group of day were provided in drinking water to the 5th group Company, Pune ). which was designated as T5.Venlyte (M/S Venky average relative humidity was 67% for the month of Pharmaceutical of Venkateswar Group of Company, May and 78% for the next month.Mean and standard Pune) was given @ 10g in 4 lits of water to the birds error of haematological traits of broiler at 6th week of belonging to the 6th group known as T6.
age in different treatment group are presented in table-In case of broiler, haematological parameters 1.Haemoglobin concentration varied from 8.317g to such as Haemoglobin concentration in the blood, total 9.53g per dl of blood in different treatment groups.The control group T1 had a Haemoglobin content of erythrocyte count and total leucocyte count were 8.9g/dl of blood did not differ significantly from the performed at sixth week of age.Same parameters were studied in broiler breeder hen after the end of the groups fed with Zeetress (T4), Stressroak (T5) and experimental period.In case of broiler, biochemical Venlyte (T6).All these four groups had significantly parameters such as total blood glucose, total serum more Haemoglobin than the group fed with vitamin C protein, total serum albumin, total serum cholesterol, (T2).total alkaline phosphatase(ALP) activity were The RBC count per µl of blood varied from performed at sixth week of age.In broiler breeder hen, 2.1075 to 2.8545 million in different treatment groups.total blood glucose and total serum protein were The group receiving vitamin C had lowest RBC count, performed at the end of the experimental period.The although it did not differ significantly from the groups meteorological data collected from the University receiving Zeetress (T4), Stress roak (T5) and Venlyte Meteorological centre.
(T6).The total WBC count was found to be significant The data generated were subjected to statistical between treatments.Sodium bi carbonate group (T3) analysis according to Snedecor and Cochran (1967) had highest WBC count followed by T1, T5, and T6. for calculation of mean, standard error and making The group which received vitamin -C (T2) had least analysis of variance.When differences between the number of WBC among the groups.Moye et al (1969) treatments were found to be significant statistically, and Vo et al (1978) however found no variation in the DMR test (Duncun, 1955) as modified by Kramer RBC count due to temperature in case of males of poultry.( 1956) was followed for separating the treatment Mean and standard error for biochemical traits of means.
broilers of 6th week of age in different treatment groups presented in table-2.Total blood glucose was

Results and Discussion
measured in mg/100ml of blood and was found to vary The maximum temperature during the month of from 94.18 mg/100ml to 113.46 mg/100 ml in May, varied from 36.4 ºC to 44.7ºC and the average different treatment groups.Differences between the maximum temperature was 39.5 ºC.During the month treatment groups were found to be highly significant. of June, maximum temperature ranged from 28.0 ºC to T2 which had maximum glucose concentration did not 42.6 ºC with the average temperature of 36.9 ºC.The differ from T4.Both these groups however differ significantly from rest of the groups for blood glucose.test revealed no significant difference among the DMR test also revealed no significant difference groups receiving vitamin-C (T2), Sodium bi carbonate between T1 and T3, T3 and T6, and T5 and T6.(T3), Stressroak (T5) and Venlyte (T6).The total RBC Total serum protein was estimated in g/100ml of count varied from 2.70 to 2.98 million per µl of blood in different groups with statistically no significant serum and varied from 2.162g to 4.5g in different treatment groups.T6 which had the highest serum differences amongst the groups.The leucocyte count protein concentration did not differ from T4 whereas of blood ranged from 27,373 to 29,266 per ml of blood both these groups were found to be significantly in different treatment groups.Differences between different from rest of the groups.Total serum albumin treatment groups were not significant statistically.concentration was highest for T6 followed by T4, T2, Mean and standard error of biochemical traits T5, T3 and T1 in that order.DMR test revealed no measured in broiler breeder hen is presented in table-4.significant difference between T6 and T4, T5 and T2, The total blood glucose level ranged from 171.75 to and T3 and T1.
186.49 mg/100ml of blood.T1 which had least total Total alkaline phosphatase activity was blood glucose level did not differ significantly from measured ml U/ ml/ min of serum.DMR test revealed T2, T4 and T5.Differences between T2, T3, T4, T5 no significant differences among Venlyte, Zeetress, and T6 were not found to be significant.Total serum vitamin -C and control groups.Sodium bi carbonate protein concentration ranged from 5.46 to 6.22g/100 group had least alkaline phosphatase activity of 31.43 ml of serum in different treatment groups.Analysis of ml U/ml/min of serum.
variance revealed no significant difference between Total serum cholesterol was measured in mg/dl the treatments. of serum.It varied from 65.54 to 109.81 mg/dl of