Influence of diet supplementation with Saccharomyces cerevisiae on intake and nutrient utilization in Graded Murrah buffaloes

A feeding trial was conducted in graded Murrah buffaloes to study the influence of yeast culture (Saccharomyces cerevisiae CNCM I-1077 strain) supplementation on intake and nutrient utilization. 12 graded Murrah buffaloes with an average body weight of 465.4 ± 20.92 kg were randomly divided into two groups (Control and treatment) of 6 animals each. Animals in both the groups received a basal diet comprising of roughages and concentrates separately to meet the maintenance and production requirements (ICAR, 1998). In addition, the animals in treatment group received yeast culture @ 0.5 g/animal/day. The average DMI of buffaloes during the digestion trial was 114.31 and 119.24 g/kg W0.75 respectively, in control and treatment groups. The digestibility coefficients of gross nutrients and fibre fractions showed non-significant differences between the control and treatment groups, though the values were found to be comparatively higher in the yeast supplemented group. The DCP and TDN contents were observed to be 8.03 and 53.06 per cent in control group and 8.15 and 54.06 per cent in treatment groups, respectively. It can be concluded that yeast culture did not show any significant positive effect on nutrient utilization in graded Murrah buffaloes.


Introduction
randomly divided into two groups (Control and treatment) of six animals each.All the animals were Yeast cultures have been fed to dairy cattle for kept in well ventilated shed with cemented floor and more than 60 years with varied response.In some were dewormed before the start of the experiment.studies, yeast cultures improved dry matter intake Animals in both the groups received a basal diet (Williams et al., 1991;Wohlt et al., 1991) and milk comprising of roughages and concentrates separately production (Williams et al., 1991;Wohlt et al., 1991; to meet the maintenance and production requirements Piva et al., 1993), whereas other studies (Erdman and (ICAR, 1998).Concentrates were offered twice daily Sharma, 1987; Arambel and Kent, 1990; Soder and at the time of milking and chopped hybrid Napier Holden, 1999) found no response to yeast cultures.
(APBN-1) was offered ad libitum.The animals in Similarly, in some studies, yeast cultures improved the treatment group received yeast culture (Saccharomyces digestibility of all nutrients (Reddy and Bhima, 2003; cerevisiae CNCM I-1077 strain) @ 0.5 g/animal/day.Kumar and Reddy, 2004;Kumar and Ramana, 2008), The yeast culture was administered by top dressing whereas in other studies, no change in total tract over concentrate mixture every day.Clean drinking digestibility was observed by yeast feeding (Kamra et water was offered to all the animals ad libitum during al., 2002;Mruthunjaya et al., 2003).There are many the entire period of the experiment.After 120 days of factors which influence the effect of yeast such as feeding, a digestion trial was carried out with an environment, nutritional status, mode of feeding, type adaptation period of 2 days and collection period of 6 of strain used etc.Therefore, the present experiment days.was conducted to study the efficiency of selected yeast All the animals were weighed before the start culture (Saccharomyces cerevisiae CNCM I-1077 and after the experiment for two consecutive days and strain) as a probiotic in Graded Murrah buffaloes.
the average values were recorded as the actual body

Material and methods
weight.During the digestion trial, the amount of feed (chopped hybrid Napier and concentrate mixture) Twelve graded Murrah buffaloes with an offered, residues left and faeces voided was recorded.average body weight of 465.4 ± 20.92 kg were Daily representative samples of the feeds, residue and of fibre fractions were higher (P>0.05) in buffaloes fed faeces were collected and pooled animal wise.The with yeast culture as compared to the control group samples were analyzed for the proximate principles as confirming the earlier reports of Garg et

Results and discussion
(2009) reported non-significant increase in DCP (%) The chemical composition of feeds and fodders and TDN (%) fed yeast culture in the diet whereas used in the present study is shown in table 1.The Kumar and Reddy (2004) reported significant increase average DMI of buffaloes during the digestion trial in DCP (%) and TDN (%) content fed yeast culture in was 114.31 and 119.24 g/kg W0.75 equivalent to 2.49 the diet.The DCP intake in terms of g / kg W0.75 and and 2.57 kg/100 kg BW, respectively, in control and kg / 100 kg BW was found to be 9.16 and 0.20 and 9.73 treatment groups (Table 2).Although differences in and 0.21 in control and treatment groups, respectively, dry matter intake between the two groups were non-which were higher than the requirement (6.76 g / kg significant but an improvement in intake was observed W0.75) suggested by ICAR (1998).The TDN in terms on yeast supplementation which may be partly due to of g / kg W0.75 and kg / 100 kg BW was found to be improved rate of fibre breakdown (Martin and Nisbet, 60.44 and 1.29 and 64.52 and 1.37 in control and 1992) and partly by improved duodenal flow of treatment groups, respectively.absorbable amino nitrogen.Similar results were The probable reasons for the contradictory observed by Garg et al. (2000) who reported increased findings can be explained by the difference in the level of yeast culture used.Many researchers felt that unless DMI in HF cows by feeding yeast culture in the diet.the level is not adequate, probiotic will not have any On the contrary, Phondba et al. (2009) reported beneficial effect on the host.decreased DMI in crossbred cows fed yeast culture in It is concluded that yeast culture did not show the diet.The digestibility coefficients of dry matter, any significant positive effect on nutrient utilization in organic matter, crude protein, ether extract, crude fibre graded Murrah buffaloes.and NFE (Table 2) showed non-significant differences between control and treatment groups, though the Acknowledgement values were found to be comparatively higher in the The authors are thankful to the Director of yeast supplemented group.The improvement in Research, SVVU, Tirupati for granting permission to digestibility could be due to increased fibre carryout the research work at Buffalo Research station, breakdown (Dawson et al., 1990) al. (2009).per AOAC (1997) and cell wall constituents as per Van The DCP (%) and TDN (%) content were Soest et al., 1991.The data generated were subjected observed to be 8.03, 53.06 and 8.15, 54.06 in control for the test of significance (Snedecor and Cochran, and treatment groups, respectively.However, no 1976).significant difference was observed between the two groups.Similarly, Garg et al. (2009) and Phondba et al.

Table - 1: Chemical composition (% DMB) of feedstuffs
Influence of diet supplementation with Saccharomyces cerevisiae in Graded Murrah buffaloes