Effect of cow urine on wound healing property in Wister Albino Rats

The wound healing activity of cow urine was studied in Wistar albino rats. The efficacy of wound healing property was evaluated by excision wound model. The parameter studied was the rate of wound contraction. The studies on excision wound healing revealed that all the groups showed decreased wound area from day to day. On day 4th, the external application of urine showed significant increase in wound healing in male and female rats compared to all other groups. th However, on 14 post wounding day, Group I and VIII animals showed 0 % and 0.40.4 % of healing was left, which may be due to normal immunity of the animals. Where as nitrofurazone treated animals showed 0 % and 0.50.3 % healing .The study revealed that the cow urine on external application to the wound, hastened the wound healing process.


Introduction
and No. 6896907 to an "Indian innovation which has proved that cow urine can make antibiotics, anti-Cows were regarded as wealth and were the fungal agents and also anti-cancer drugs more backbone of the economy of ancient Indians.Cattle effective".Besides, cow urine is said to be a very were one of the most frequently used animals effective insect repellent when mixed with certain described in Vedas.Cows were regarded as mother herbs (Nair, 2002).("Gau-mata") and referred to as Aghnya.Cattle There are numerous uses of cow urine for husbandry was well developed during the Rigvedic various human ailments like, cancer, osteoarthritis, period (1500-1000 BC) and the cow (Kamadhenu) allergies, kidney failures, skin diseases, healing of was adored and considered the 'best wealth' of wounds, etc Need of hour is another revolution; mankind.Urine was also considered as an antidote to through extensive research in cowpathy for its poisons (Sushrut Samhita).
scientific validation and a wider popularityVisualizing In Sushrut Samhita, several medicinal properties the potential use of cow urine in several ailments of cow's urine have been mentioned and cow urine was including even cancer, the use of Gomutra (cow urine) known to cause weight loss and to cure leprosy, and its scientific therapeutical validation is required cardiac and kidney problems, indigestion, stomach for its worldwide acceptance and popularity.ache, edema, etc. (Kaviratna and Sharma., 1996).This The redistillate of cow's urine was found to kind of alternative treatment, termed as 'panchgavya possess total antioxidant status of around 2.6 m mol, therapy' or 'cowpathy', (Dhama, K, et al., 2008) has contributed mainly by volatile fatty acids (1500 mg/L) been reported to be beneficial even for dreaded as revealed by the GC-MS studies.These fatty acids diseases like cancer, AIDS and diabetes.and other antioxidants might cause the observed "Kamadugha Yojane" has been drawn up to protective effects (Krishnamurthi et al., 2004).Olusi protect Indian cows in appreciation of the multifarious and Ojewole (1978) revealed that oral administration uses of "Panchagavya," which comprises cow dung, of cow's urine concoction in rats causes the activation urine, ghee, curd and milk, which is found to be of the third complement component in the serum.The effective in treating major diseases such as cancer and product of this activation has some histaminediabetes.The fact that cow urine costs more than milk releasing effects and causes a characteristic acute fall speaks of its limitless medicinal use (Raghaveshwara in neutrophil and monocyte counts in the peripheral Bharati Swamiji, 2006).United States Patent and blood which is reversed within four hours.Trade mark Office had granted Patents No 6410059 .
The detailed studies on pharmacological activities university grazing land.All the animals were monitored and safety evaluation of cow urine employing carefully throughout the experimental period.controlled studies in experimental animals are scanty.
Collection of urine : Natural voiding mid-stream Hence, the present study was undertaken on cow urine was collected in a sterile glass container.o Collected urine was stored in refrigerator at 4 C and urine in male and female Wistar albino rats with the utilized for the experiment within two hours of objective to study the effect of cow urine on wound collection.Precaution was taken to avoid any external healing.
contamination Urine was collected from the same

Materials and Methods
cows and used in each experiment.Immediately after Selection of experimental animals : Wistar albino collection physical, chemical and microscopic rats procured from the Laboratory Animal Facility of examinations were done Urine specimens were collected and stored as part of standard protocols in Veterinary College, Hebbal, Bangalore, were used in cohort studies designed to assess exposure to non the present study.They were aged between six to seven persistent environmental contaminants (Gunter, 1997; weeks and weighed 150-200g.They were acclimatized Landi and Caporaso, 1997).to the experimental conditions for one week.After Selection of doses for pharmacological assays: acclimatization, rats were grouped and housed in The doses corresponding to the dose of 15 ml, 30 ml, standard polypropylene cages during the experiment.60 ml and 75 ml per day in a human being weighing They were maintained under standard laboratory around 60 kg.The doses administered were 0.05 ml, hygienic conditions, providing standard laboratory 0.1 ml, 0.2 ml and 0.3 ml/100 gm body weight in rats animal feed and water ad libitum.The approval of the (The higher range of rat's body weight was considered Institutional Animal Ethics Committee was obtained for dosage calculation).prior to start of the experiment.

Administration of doses:
The fresh urine at 0.05 ml, Selection of urine source : Holstein-Friesian (HF) 0.1 ml, 0.2 ml and 0.3 ml/100 gm was selected and was cross-bred, milking cows aged about 2-3 years were made up to 2 ml by adding distilled water and was selected for urine collection.Animals were hygienically gavaged using a stomach tube daily for a period of 14 maintained in Karnataka Veterinary, Animal and days.Fisheries Sciences University regional campus, Dept.
Six groups were made (n=6).The group I and of Livestock Production and Management, Veterinary VIII was considered as the control in male and female College, Hebbal, Bangalore.All the cows were fed rats, the group II and IX served as reference standard with the concentrate prepared at the dairy farm and ® were provided clean drinking water ad libitum and and treated with 1% (w/w) Furacin ointment, the daily animals were also taken outside for grazing in the group III -VI and X-XII animals were treated with . .samples from HF cross bred cows was screened for 60.72.7 in female rats.There was significant increase wound healing activity.In the present study during in the percentage of wound healing in external applied assessing wound healing activity, the cow urine which urine treated group (P<0.05).

Effect of cow urine on wound healing property in
has been applied externally to the wound, hastened The percentage of wound area in group VIII, IX, th the wound healing process.Further research needed X, XI, XII, XIII and XIV on 12 day was 2.60.8, to isolate and identify the active molecule/molecules 2.90.6, 3.80.6,3.30.7,3.81.0,2.30.8 and 0.00.0 in responsible for wound healing activity.female rats.There was significant increase in percentage of wound healing in externally applied