Simultaneous Infestation of a Buffalo calf with Ascaris and strogyloides : A case study

Toxocara vitulorum occurs in the small intestine of Indian buffalo, and is found in many places of world. Small intestinal infestation with Strongyloides papillosus occurs in ruminants. However, we diagnosed a nondescript Indian buffalo calf infested simultaneously with Toxocara vitulorum and Strongyloides papillosus and treated it with standard regimen and found the subject cured.


Introduction
normal range respectively.In the faecal sample examination by direct smear method , we found eggs One month old nondescript buffalo calf was of Toxocara vitulorum and Strongyloides papillosus in presented to us with a history of intermittent diarrhoea, the same microscopic field.In the centrifugal floatation poor growth, dullness and anorexia.We checked the method, however, we got the eggs of Toxocara body temperature, pulse rate, respiration rate of the vitulorum in huge number with insignificant no.of subject and found them to be normal.Then, we Strongyloides papillosus eggs.From microscopic collected the faecal sample of the buffalo calf directly examination of faecal sample of the buffalo calf we from the rectum and examined it fresh for any confirmed the condition to be a simultaneous infestation suspected gastrointestinal parasitism.
of the subject with Toxocara vitulorum and Strongyloides papillosus.

Materials and methods
Accordingly, we asked the owner of the animal

Macroscopic Examination of Faecal sample:
to bring the fresh faecal sample of the dam of the Nearly 10 g of faecal sample was directly collected buffalo calf.After examination of the faeces of the from the rectum of the subject and was examined dam we found only eggs of Strongyloides papillosus physically for any abnormality.The color of the faeces and no eggs of Toxocara vitulorum.So, we treated the is muddy, the consistency was semi-solid with streaks calf with Piperazine hexahydrate @ 300mg/Kg body of mucus and the odor of the sample was weight[Piperazine Hexahydrate solution 45% characteristically evil.
w/v(TTK) 20ml total dose] orally and with

Microscopic Examination of faecal sample:
Levamisole hydrochloride @7.5 mg/Kg body weight Samples are prepared for the microscopic examination [Lemasol-75 (Ranbaxy)3ml] subcutaneously.As of the faeces by following direct smear method and supportive therapy we administrated 1ml of Tribivet Centrifugal flotation method as per the standard (Intas Pharmaceuticals) intra muscularly on alternate protocol.The Samples were examined first under 10X days for three times to the calf .For treating the dam objective with 10X eye piece lenses and then under against Strongyloides papillosus, we administered 45X objective with 10X eye piece lenses of a binocular Fenbendazole @ 5mg/Kg body weight[Fentas 1.5g compound microscope of Ajay Optik 03-A-3076 bolus (Intas Pharmaceuticals)total dose] and 5ml of model and photographs were taken using a digital Tribivet was administered Intra muscularly on camera of Sony Cyber-Shot DSC-w120 model.
alternate days for three times to the dam as a supportive therapy.After, the first dose of piperazine

Results and Discussion
in the evening, two adult Toxocara vitulorum worms The rectal temperature, pulse rate and respiration were expelled in the faeces of the calf in the next 0 morning.Again, we measured the length of the worms rate of the buffalo calf were found 101.2 F , 60 per minute and 24 per minute which were within the and found one of the worm measured approximately 25cm.and another worm approximately 29 cm.and transmammary infections constitutes the major routes of the parasite.Finding of strongyloides eggs (figure) which conforms to the normal length of Toxocara vitulorum worms.The expulsion of the adult may be due to either percutaneous or oral route of worms reinforced the efficacy of our line of treatment.infection.Further studies regarding the interaction of After 20 days we again examined the faecal the two worms within the animal body will definitely sample of both the calf and its dam for presence of help to illuminate whether the two worms have any parasitic ova and found the samples negative for any synergistic pathogenic effect.parasitic ova.This result showed that the calf was

Figure - 1 :
Figure -1 : Egg of Toxocara vitulorum and larvated egg of Strongyloides spp. in fecal sample preparation ofthe buffalo calf.(Direct Smear method) F i g u r e -2 .H u g e n o o f Toxocara vitulorum eggs from the feacal sample of the buffalo calf: centrifugal floatation method, 10X.