Broad spectrum probiotic ( Sakhabactisubtil ) recovered from Yakutia permafrost soil

Probiotic “Sakhabactisubtil” has been developed, it has unique biological properties: frank antagonistic effect against pathogen and opportunistic microorganisms (bacteria, fungi and viruses), interferon-evoke activity, immune system enhancing effect, producing a range of ferments, beneficial microflora formation's stimulation, resistance to a number of antibiotics.


Introduction
ctisubtil" based on the strains of bacteria Bacillus subtilis "TNP-3" and "TNP-5": At present the world community gives a great • frank antagonistic action against many pathogen consideration to food products' safety.In the EU from and opportunistic microorganisms (streptococci, January of 2006 the application of antibiotics has been staphylococci, E.coli, salmonella, brucella, banned in animal breeding.Therefore last decades campylobacter, atypical mycobacteria and viruses) as there undergo development and innovation of safe and well as some of toxigenic fungi (Penicillum, effective probiotics as alternatives to antibiotics in the Aspergillus, Stachybotrus) and soil microorganismswhole world including Russia.
pathogens of fungal diseases (Rhizoctonia-solani, Development and application of ecologically Streptomyces, Fusarium oxysporum); friendly biopreparations which favor to animal health, • stimulation of beneficial intestinal microflora especially in harsh conditions of the Extreme North, is development; a topical issue in veterinary medicine.Spore-forming • complex of enzymatic activities (proteolytic, bacteria play a special place in a range of probiotics. gelatinase, amylase, cellulose, β-gluconase and Probiotics' safety in producing is a reason to study marked xylanase and fructoziltransferase), which them as a preventive and treatment preparations intensify antagonistic action and favor to more frank during different pathologic processes in humans and animals (B.R. Tarakanov, 1987; P.I.Jdanov, 1997; probiotic effect; I.I.Bratukhin, I.N.Jirkov, 1999).
• resistance to a wide range of antibiotics, as a In the recent decades the Yakut Scientific result of it there is a possibility of application in Research Institute of Agriculture has developed a combination with antibiotics and the preparation is range of innovative probiotic preparations on the basis also recommended after serious illnesses, antibiotic of biologically active and unique local natural strains treatment, with rapidly eliminated dysbacteriosis of bacteria Bacillus subtilis as "Sakhabactisubtil" intestine.(approved by the Federal Service for Veterinary and Moreover, the preparation is active inducers of Phytosanitary Surveillance of the Ministry of endogenous interferon and enhances immunological Agricultural Sciences of the Russian Federation, reactivity.It adjusts metabolism, improves feed 14.11.2006).Bacillus subtilis strains "TNP-3" and digestion and assimilation and contributes to body "TNP-5", recovered from permafrost soil, have been weight growing.certified and deposited to the All-Russian micro-Prevention and treatment of newborn organisms' collection used in animal breeding and calves' and piglets' dysbacterioses veterinary as having frank biological active properties.
The application of preparation favors to regular Biological properties of preparation "Sakhaba-intestinal microflora formation, except it amounts of microflora and restores a reproductive ability.It bifidus bacteria, lactic acid bacteria and spore-forming preserves cow healthy, and it in turn contributes to bacteria are multiplied, content of lastose-negative producing environmentally safe livestock.microflora is considerably reduced, indices of natural Prevention of animals' mycotoxicosis organism resistance are increased (increasing of blood serum bactericidal, lysozyme activities, range of "Sakhabactisubtil" can be used in feed to prevent globulin fraction, blood phagocytic activity), sickness fungal toxins.Can be Fed with fodder infected by rate is reduced, losses out of diarrhea are prevented.molds also.Cattle are treated with the preparation at a In the prevention of dysbacterioses newborns are dose of 50 billion CFU per 1 head orally once a day draughted orally 10 ml (1 dose) once a day for 3 during 30 days.consecutive days after their birth.
The preparation has a frank antagonistic activity With the purpose of treatment the preparation is against toxigenic mold (Penicillium, Aspergillus and Stachybotrus).It improves an immunological prescribed in 10 ml 3 times a day until the reactivity of organism and normalizes intestinal disapparance of clinical signs of disease.If necessary, microbiocenosis."Sakhabactisubtil" can be given in combination with other medicines (antibiotics, sulphonamide, etc.).The Prevention of metabolic disorders in same dose of preparation is prescribed for immunity cattle enhancement once a day for 10 consecutive days.Preparation "Sakhabactisubtil" has no side effects on Mineral and vitamin supplement containing animals, even in the case of overdosing.Preventive "Sakhabactisubtil" with a frank enzymatic activity is use of the preparation protects newborn animals from used in prevention of methabolism.diseases and its rate is 96-100% and preservation is It can be used daily at a dose of 50 billion CFU 98,5-100%.Beneficial effects of the preparation is during the last 2 months of pregnancy.Mineral and 81,8-100%.Rate of diarrhea incidents decreases, rate vitamin supplement with preparation "Sakhabactisubtil" of death reduces, immunity and resistance to intestinal provides a daily requirement of vital micronutrients, diseases increase, body weight gaining of newborn vitamins, and stimulates physiological and animals is significantly improved.biochemical processes in the body.Moreover, it increases a digestibility of nutrients' intake by Prevention and treatment of herd available enzymes and increases productivity.The horses' respiratory diseases application of mineral and vitamin supplement with preparation "Sakhabactisubtil" improves immunity "Sakhabactisubtil" can be applied in treatment of and enhances enzyme activity.It stimulates horses.The doses of preparation can be injected to a biochemical processes and normalizes intestinal nasal cavity by a draught of 5 ml doubly in 10-12 days.microflora.Thereby it contributes to the prevention of Preparation "Sakhabactisubtil" has a detrimental metabolic disorders and improves productivity.effect on the causative agent-Streptococcus equi."Sakhabactisubtil" improves immunity that has a Sanitation of ice-houses of fur frank therapeutic effect and it shortens recovery.

animals' fodder storing Prevention of cows' postpartum complications
Preparation "Sakhabactisubtil" can be used for The preparation can be used in immunity the sanitation of ice-houses at a dose of 1 billion 2 improvement of cows in calf and correction of vaginal CFU/ml at 100 ml/m .Solution of the preparation is microbiocenosis.
applied by fogging.Preparation "Sakhabactisubtil (50 billion CFU) The biological method of ice-houses' sanitation can be used with forage to 5 g during 10 days 3 using preparation "Sakhabactisubtil" has showed high consecutive months; The culture liquid of bacteria efficacy against mold.The application of preparation Bacillus subtilis strains "TNP-3" is injected restrains the development of toxigenic fungi and hypodermically at a dose of 2 ml once in 10 days 3 Aspergillus, Mucor mold in ice-houses up to 3 months.times a month; The preparation "Sakhabactisubtil" is Preservation of silage by addition of a injected intravaginally after a birth at a dose of 10 ml microbial preparation (50 billion CFU) daily for 3 days.
Genital organs' microbiocenosis correction of Preparation "Sakhabactisubtil" can be added to cows with probiotic preparations normalizes preserve silage at a rate of 100 ml containing 1 billion CFU / ml per 1000 kg of silage.The preparation has production is certified and organized under microbial not only antagonistic property against many preparations' development laboratory of the Yakut pathogens, but also a complex of enzymatic activities Scientific Research Institute of Agriculture.(proteolytic, gelatinase, amylase, cellulose, β-