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Research
7.
Protein profile of Chlamydophila abortus
isolates from Kerala, India -
Binu K Mani, M Mini, V N Vasudevan
Vet World. 2011; 4(10): 470-472
doi: 10.5455/vetworld.2011.470-472
Abstract
Chlamydiae are of microbiological interest because
of their mode of interaction with eukaryotic host
cells and their specialized life cycle with unique
features of parasitism. Reports regarding
prevalence of infections of Chlamydophila abortus,
the causative organism for chlamydial abortions in
livestock, was the basis of the study. Two
isolates, one each from cattle and goat abortion
along with a reference isolate, were used for
characterization with Sodium Dodecyl Sulphate-Poly
Acrylamide Gel Electrophoresis (SDS-PAGE).
Elementary bodies infected Mc Coy cells, harvested
from bottle cultures were disrupted by Teflon
coated magnetic pellet. Urografin-76 diluted with
Tris-Potassium hydrochloride was used for
purification of Elementary bodies of Chlamydophila
abortus organism. On protein estimation of
Elementary bodies by Biuret method, all the three
isolates revealed protein concentration between
500-1000 mg/100ml, which were sufficient for
electrophoresis. Ten percent of resolving gel and
five percent of stacking gel of polyacrylamide in
which 10g of processed isolate samples along with
standard protein marker and Mc Coy cell protein
(control) were electrophoresed. Using Alpha Imager
Gel Documentation System, the protein bands were
analyzed. Twelve bands each for local bovine
isolate and reference isolate were noticed while
only 10 bands were there in the caprine isolate.
Additional bands of 148 kDa and 135 kDa were
present in bovine isolate, compared to the
reference isolate, while 152 kDa and 137 kDa bands
were unique for caprine isolate.
Keywords:
Chlamydophila abortus, protein profile, Mc Coy
cell, elementary bodies
