Comparision of Prestokon and Furazine-II in the treatment of Microbial diarrhoea in neonatal lambs

® ® A comparative efficacy of Prestokon and Furazine-II in the treatment of microbial diarrhea in neonatal lambs over a 24 hour period was evaluated. Microbial Colony Forming Units (CFU) per gram of each faecal sample was determined before treatment and 24 hours after treatment, specific causative microorganisms were not isolated. The average total ® ® CFU / g of faeces killed within 24 hours by Prestokon was 63.31+ 6.22 % and 32.47+14.75 % for Furazine-II . The ®. clinical response to treatment was also significantly (P < 0.01) better in lambs treated with Prestokon Although the ® result of this study suggest that Prestokon might be more effective in the treatment of microbial diarrhea in lambs, it must be pointed out that clinically both drugs can be given repeatedly over a period of several days until clinical cure or improvement was observed.


Introduction
aged 7 -21 days presented to the Animal Health Unit of the Institute showing clinical signs of diarrheal New or improved methods of preventing or disease were randomly assigned to two treatment treating diseases are being tested continually in an groups of seven each.Lambs in-group A were treated effort to improve the health and productivity of animal  to compare the efficacy of Prestokon and Furazine-II was considered good if diarrhea had ceased 24 hours after the first treatment and there was significant in the treatment of microbial diarrhea in neonatal lambs.
decrease in the total bacterial count per gram of faeces.
. faecal sample before treatment and 24 hours after 1gm was aseptically weighed from each faecal sample and dissolved in 9ml Physiological treatment.There was an interval of 12-48 hours -1 between collection of the samples and culturing for Saline Solution (PSS) to give a dilution of 10 .This -6 microbial colony forming units.Culturing the faecal was serially diluted to 10 , and using sterile pipettes -4 -6 0.1ml of the 10 and 10 dilutions were surface plated samples within 24 hours of collection probably would on different plates of Nutrient Agar.They were have resulted in a higher CFU /g before treatment, o because the number of some microorganisms incubated for 24 hours at 37 C and the colony forming units, CFU / g of each sample determined.
decreases rapidly as the sample ages (De, et

Results
prominent amongst which is Neomycin sulphate, a The results of this trial are presented in Tables 1 medium spectrum antibiotic used widely for coliform and 2. The average total CFU / g of faeces killed within enteritis in all species of animals (Scheifele et al., ® 24 hours by Prestokon was 63.31+6.22 % and 32.47+ 1987).Also with Scopolamine Hcl, a parasympatho-® 14.75 % for Furazine-II .Similarly, clinical response lytic compound that abolishes tone and causes to treatment was significantly (P < 0.01) better in relaxation of the smooth musculature of the ® lambs treated with Prestokon than those treated with gastrointestinal tract thereby reducing the intensity ® and frequency or rate of progression of peristalsis that Furazine-II .However none of the lambs died during accelerate transit of ingesta and alimentary secretions the experimental period and those with diarrhea at the especially that they are both gut active and not 999-1003.5.
destroyed by milk.The relatively lower efficacy of

®with
Prestokon suspension and those in group B with populations (Williamson, 2002; Constable, 2004; ® Furazine-II water suspension.Schoenian, 2009) Disease problems such as microbial Selection of cases: The criterion for clinical infection and endo-parasitism often result in diarrhea, diagnosis of diarrheal disease was semi fluid-towhich has a devastating effect on production and watery faeces and having a rectal temperature above productivity (Radostitis et al., 2007).Neonatal diarrhea the normal range (102-103°F

10 days of age, although it may Treatment regimen: Diarrheic lambs were treated occur in older animals (Nóbrega Júnior et al
Furazine-II at the rate of 1ml/kg of 2008).Diarrheal disease in lambs has received body weight was given orally to lambs in Group B. All relatively little attention compared to calves and piglets where extensive investigation has been done lambs that were still diarrheic 24 hours after the first ® of body weight.

®
Comparision of Prestokon and Furazine -II in the treatment of Microbial diarrhoea in neonatal lambs 60mg suspension containing: Neomycin sulphate 1g, end of 24 hours after the first period were treated again thalysulphathiazole 6g, Spolamine Hcl 0.005 g, until clinical cure or improvement was observed.Vitamin A palmite 50000 iu, Vitamin E 0.4g. Bacteriology: al., 2002; Statistical analysis: The means and standard Orden, et al., 2007; Francisco and Songer, 2008).deviations of each CFU / g of faeces before treatment Results of this study indicated a higher ®and 24 hours after treatment were calculated.The percentage of CFU / g of faeces killed by Pretokon paired t-test method was used to determine level of clinically after one treatment.This might be due to the significance(Steel et al., 1997).compositionand mechanism of action of the drug.Prestokon is made up of 5 active ingredients,

Table - 2. Response of Diarrhoeic lambs to Furazine-II Treatment (F = Furazine-II) ®
Comparision of Prestokon and Furazine -II in the treatment of Microbial diarrhoea in neonatal lambsTakahashi, T. (2005).Antimicrobial Susceptibility of through the intestines.The combined effects of these Pathogenic Escherichia coli Isolated from sick Cattle and compounds appear to have a higher bactericidal effect Pigs in Japan.Journal of Veterinary Medical Science 67(10):