Effect of Different Housing Systems on Triidothyronine (T) 3 and Thyroxine (T) in Chhotanagpuri Ewes 4

The present study was conducted on 24 non-pregnant parous chhotanagpuri ewes to see the effect of different housing system on the level of Triidothyronine (T ) and Thyroxine (T ) hormones. Animals were randomly 3 4 allocated to three groups, one control and two treatment. Animals in Group I were kept in hot humid condition where as Group II was maintained in cold condition providing gunny bags and fine sand on the floor. Control animals (Group III) were kept in loose housing system fodder and water were provided ad. libitum. to all the three groups of animals. Level of T3 and T4 hormones were estimated during different stages of gestation. Result of present study revealed that the level of plasma Triidothyronine (T ) varied significantly within the groups in all 3 the groups while it differed significantly (P<0.05) only at day 135 of gestation between groups however, it increased significantly on expected day of parturition in all the three groups. The plasma Thyroxine (T ) level 4 varied significantly (P<0.05) within and between groups at all the stages of gestation. The plasma Thyroxine (T ) 4 level decreased significantly from day 0 to day 45 and then started increasing up to day 135 of gestation in all the groups. Further the, plasma Thyroxine (T ) level decreased significantly (P<0.05) on expected day of parturition 4 and again increased two days after parturition in all the groups but it was non-significant. Different housing systems did not had any effect on T3 and T4 hormone levels, except at day 135 of gestation.


Introduction
Pregnancy or gestation is one of the important periods of any living organism.It depends mainly Sheep population in India is around 49.20 on the level of hormones and adequate nutrition.million (according to 1992 census).The country These factors control the normal status of pregnancy stands sixth in sheep population in the world.The and so we must know their relative importance in sheep produce 141 million kg mutton 35 million the period of gestation.Reproduction is closely kg greasy wool and 39.78 million pieces of skin associated with interaction of hormonal and annually (Acharya, 2002)

Results and Discussion
Seed Project", monitored by ICAR.Animals were randomly divided into three groups, one The mean values of T3 and T4 hormone control and two treatments respectively.Animals have been depicted in table I and II.The results in the three groups were offered green fodder i.e. showed that the level of triidothyronine increased Maize, oat, berseem with addition of concentrate continuously in all the groups from day 20 to two mixture @250 gm/head daily to all experimental days after parturition.Analysis of variance animals.Fenbendazole @10mg/kg body weight showed significant (p<0.01)effects of housing was administered to all animals before the start of systems within the groups on the level of experiment.To determine the level of Triidothyronine triidothyronine.The level of T3 during mid of (T ) and Thyroxine (T ) hormones, 2ml blood was 3 4 gestation remained almost similar in all the three collected in a heparinised sterile vaccutainer.The groups, but after two days of parturition there was blood samples were collected at different intervals significant (p<0.01)variability in T3 level in the i.e.Day 0, 20, 45, 90, 135, Expected day of blood plasma of chhotanagpuri ewe.The level of Parturition and two days after parturition.Plasma T after lambing in all the three groups increased 3 was separated by centrifugating it at 3000 RPM simultaneously with no significant changes.for 15 minutes and stored at -20°C in deep freeze.
These findings do not agree with the report of The plasma sample were carried to IVRI, Prakash and Rathore (1991) who reported that Izatnagar for estimation of T (Lerman, 1953) and high ambient temperature depress the activity of 3 T (Marsden, 1975) . Having the knowledge nutritional status of body. of hormones particularly reproductive hormone, we can diagnose whether animal is pregnant or Materials and Methods not and if they are pregnant what is the stage of To cite this article : Verma RK, Ishwar AK, Sinha MP and Bharti A (2012): Effect of Different Housing Systems on Triidothyronine (T ) 3 and Thyroxine (T ) in Chhotanagpuri Ewes, Vet.World 5 (2): 100-102, doi: 10.5455/vetworld.2012.100-102

Table - 1. Mean ±SE of Plasma Triiodothyronine (T ) ng/ml of chhotanagpuri ewes in different Groups 3 at different periods.
by Radio Immunoassay kit thyroid gland.The results of experimental study A

Table - 2. Mean ±SE of Thyroxine ( T ) ng/ml of chhotanagpuri ewes in different Groups at different 4 periods. stages
of gestation.The mean thyroxine level in of Hormonal Profiles and other facilities needed.group II (55.95±0.76ng/ml) was highest as