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Original Research
8.
Screening of poultry samples for Salmonella
Typhimurium by PCR assay -
V K Anumolu
and V R Lakkineni
Vet World. 2012; 5(3): 169-172
doi:
10.5455/vetworld.201
2.169-172
Abstract
Poultry samples viz., cloacal swabs, egg swabs,
poultry faeces and feed were screened for
Salmonella Typhimurium. A set of primers
derived from fli C gene were employed to
standardize PCR for detection of Salmonella
Typhimurium from poultry samples, which gave
specific amplification of a 620 bp fragment.
Boiling and snap chilling method used for template
preparation. Screening of 112 samples revealed
that 12 samples positive for Salmonella
Typhimurium by PCR assay.
Keywords: Poultry
samples, fli C gene, PCR, Salmonella
Typhimurium
