Veterinary World


ISSN (Online): 2231-0916


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Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Original Research

8. Screening of poultry samples for Salmonella Typhimurium by PCR assay - V K Anumolu and V R Lakkineni
Vet World. 2012; 5(3): 169-172

Poultry samples viz., cloacal swabs, egg swabs, poultry faeces and feed were screened for Salmonella Typhimurium. A set of primers derived from fli C gene were employed to standardize PCR for detection of Salmonella Typhimurium from poultry samples, which gave specific amplification of a 620 bp fragment. Boiling and snap chilling method used for template preparation. Screening of 112 samples revealed that 12 samples positive for Salmonella Typhimurium by PCR assay.

Keywords: Poultry samples, fli C gene, PCR, Salmonella Typhimurium