Materials and Methods

The study was done to understand the preservation of Washed Spermatozoa of Mehsana Buck at Refrigeration temperature. 0 The washed spermatozoa from 78 semen ejaculates, 26 each from 3 Mehsana bucks, were preserved at 4 ± 1 C temperature in SM and TCFEY diluents up to 72 hours and the physical characteristics of spermatozoa were studied to assess the suitability of diluents. The individual sperm motility and live spermatozoa decreased significantly whereas abnormal spermatozoa increased significantly (P<0.05) at each 12 hrs interval of preservation in two diluents. The effect of diluents on spermatozoan characteristics was less pronounced in SM than TCFEY diluent. Among the interaction studies, stage of preservation affected the live spermatozoa and their morphology. The findings suggested superiority of SM diluent over TCFEY for preservation of Mehsana buck semen.


Artificial Insemination is a major concern in
The research protocol was conducted with a progeny testing programme of small animals which prior permission of Institutional Animal Ethics are generally reared in small geographical areas.The Committee, College of Veterinary Science, Sardarsemen cooled to low or ultra low temperature has to be krushinagar Dantiwada Agricultural University, utilized for this purpose, however, the resultant Sardarkrushinagar.Three Mehsana bucks of breeding fertility remains higher in Norwegian dairy goats by age, 12 to 18 months, were trained on teaser of same 0 breed for two months to ejaculate the semen in using semen stored at 4-5 C in comparison to frozen Artificial Vagina; and there after utilized to obtain 26 (Leboeuf et al. 2000 andPaulenz et al, 2005).These semen ejaculates from each buck twice weekly during workers have reported the Skimmed milk and Tris-egg the period of three months.yolk diluents for preservation of buck semen.The A total of seventy eight ejaculates of acceptable beneficial effect of washing on viability of spermatoquality were divided in two equal aliquots immediately zoa of crossbred bucks (Beetal x Assam local) and its after collection and each of them was washed to storage ability up to 72 hrs of preservation at refrigeremove the coagulating enzyme using Krebs-Ringerration temperature has been reported by Islam et al.
Phosphate-Glucose solution @ of 1: 9 followed by (2006) while; Ranjan et al. (2009) documented the centrifugation at 3000 rpm for 5 min (Chemineau et similar benefits in Marwari buck semen extended in al., 1991).The supernatant was removed to obtain the diluents containing 10% egg yolk.However, there sperm pack which was diluted in two different dilutors: does not seem any information on Mehsana Buck.
Tris -Citric acid -Fructose -Egg yolk (TCFEY) and Therefore, the present investigation on preservation Skim Milk (SM) at the dilution ratio of 1:10 (Deka and of Mehsana buck semen at refrigeration temperature Rao, 1986, Corteel, 1974).The samples were preserved vis-à-vis physical characteristics of spermatozoa was 0 undertaken to evaluate the suitability of SM and at 5 C temperature and were evaluated after 12, 24, 36, TCFEY diluents.
48, 60 and 72 hrs of preservation.The data were analyzed by factorial CRD having 3 factors (Snedecor motility, live spermatozoa and morphological abnormalities differed significantly due to these two variances and Cochran, 1980).
whereas live spermatozoa remained unaffected among the bucks (Table -2).Almost similar findings have The data pertained to individual sperm motility, been reported by earlier works in bucks (Mishra et al., live spermatozoa and their abnormalities at different 1993; Puranik et al., 1994) and Patanwadi rams hours of preservation in TCFEY and SM diluents are (Kakdiya, 1993).presented in Table 1.The findings showed significant The comparative findings of two dilutors revealed decrease in individual sperm motility and live the significant differences in all three spermatozoon spermatozoa at all stages of preservation in both characteristics (Table -1).The superiority of SM diluents.These two traits of spermatozoa were diluent over the TCFEY diluent was reflected at all observed to curtail more than 25 percent at 72 hrs of stages of preservation in our study (Table -1), which preservation in comparison to their initial values.corroborates with the earlier findings in Osmanbadi Other workers have also reported a considerable and crossbred buck semen as reported by John and decline in live spermatozoa and initial sperm motility Raja (1973); Das et al.(1985) and Puranik et al. in TCFEY and SM diluents (Das et al.., 1985, Mishra (1994).However, the findings of Singh et al. (1980( ) et al.1993( , Takerkhede et al. 2005).On the other hand, suggested higher sperm motility in buck semen using the morphological abnormalities of spermatozoa egg yolk citrate.This variation might be attributed to increased linearly and reached beyond the acceptable the breed characters of buck used in different studies limit at 72 hrs of preservation to both diluents.Simon besides the holding time of washed spermatozoa as et al.

(2003) also documented an increased percentage suggested by Islam et al. (2006). of sperm abnormalities as the duration of preservation
The sperm abnormalities were found to be less in SM diluent than the TCFEY at each stage of advanced.
preservation.The differences were progressive with a The effect of diluents and bucks were variable on range of 1-4 percent during the entire period of semen spermatozoon characteristics.The individual sperm

Table - 2: Analysis of variance for spermatozoan characteristics in TCEFY and SM diluents during refrigeration preservation in Mehsana bucks.
* Significant at 5% level NS = Non-significant