Multiple organs metastatic mesothelioma in a White Tiger 1 2

Aim: An attempt has been made to know the cause of the death of the female white tiger, who was considered as Queen of the Zoo and has given birth of 22 white tigers in her life time. 
 
Materials and Methods: Complete clinical examinations were carried out such as heart rate, respiratory rate and body temperature and haematology etc. After death, the detailed and complete post mortem examination was performed and representative tissue samples were processed for histological investigation by formalin fixation and paraffin embedding technique as described by Luna. 
 
Results: Mesothelioma is a form of neoplasm having mesenchymal cell origin. In this investigation clinical signs were recorded and Clinical examination revealed no abnormality except anaemia with Neutrophilia and Lymphocytosis. At necropsy, the thoracic and abdominal cavities contained a large amount of serosanguineous and blood tinged fluid. The peritoneum showed Multiple, grayish – white nodules of varying size of 1 to 2cm diameter were scattered throughout surfaces. Neoplastic growths were also found in the parenchyma of the liver, lungs, spleen, intestine and mesenteric, tracheobronchial, mediastinal lymph nodes. Microscopic examination revealed Pleural and peritoneal surfaces were markedly thickened by multiple layers of neoplastic mesothelial cells with well supported by collagenous connective tissues. The neoplastic cells of different organs (lungs and liver) showed larger vesicular nuclei, prominent nucleoli and neoplastic cell emboli. 
 
Conclusion: Gross lesions and histopathological studies were suggestive for malignant mesothelioma and originated from the peritoneum with secondary metastasis to other organs and lymphnode.


Introduction
2007.Sickness of the animal was reported to the centre for wild life, Bhubaneswar with clinical history of Mesothelioma are neoplastic proliferation of the depression, anorexia, and emaciation.It was kept in serous lining mesothelial cells of the thoracic, special enclosure for the better treatment.The clinical abdominal and pericardial cavities [1].It is a rare form examinations such as temperature, heart rate, of cancer that develops from transformed cells originating respiration rate were recorded.Observation of visible from the mesothelium.Spontaneous malignant mesomucus membrane and body coat was done to find out thelioma appears to be very rare but sporadic cases anaemic status, dehydration level and presence of have been reported in various animal species [2,3].external parasitic infestation.Faecal sample test was The present paper documents the occurrence of a rare also conducted to find out any internal parasite case of peritoneal mesothelioma with multiple organ infestation.In haematological examination, Blood metastases in a female white tiger.
smear both (dry and wet) were taken to examine for blood protozoan parasites.The tiger was treated with

Materials and Methods
fluid therapy (RL) and broad-spectrum antibiotics The subject of this study was of an eighteen years with parenteral administration of multivitamins and old female white tiger (panthera tigris) in the steroids.In spite of the intensive care, fluid therapy Zoological Park, Nandanakanan, Odisha in the year and other symptomatic treatment the tiger died just   Microscopic examination of H & E sections of serous cavities, especially peritoneum with secondary different organs revealed glandular tubuloalveolar metastasis to other organs and lymphnode.The pattern of growths lined by cuboidal cells to low mesothelioma is very rare and sporadic among columnar types with eosinophilic granular cytoplasm animals.To our best knowledge, this is the first report [1,5].Pleural and peritoneal surfaces were markedly of multiple organs metastatic mesothelioma in a white thickened by multiple layers of neoplastic mesothelial tiger under captivity in India.cells with well supported by collagenous connective metastasis of the neoplastic growths but we have arrangement with less no of mitotic figures were 6.
Harbison, ML and Godleski (1983) 0 one week after the treatment started.A complete post (100.5 F), heart rate was 60/min and respiration rate mortem examination was performed.Representative was 32/ minute.Haematological examination revealed tissue pieces from various areas of the tumour were anaemia with 8.5g/dl and PCV only 28%.Total leukocyte collected in 10% neutral buffered formalin embedded count revealed Neutrophilia and Lymphocytosis. in paraffin and cut at 5µm thickness [4].Sections were At necropsy, the thoracic and abdominal cavities stained with routine haematoxylin and eosin.contained a large amount of serosanguineous and blood tinged fluid.On examination of the abdominal Results cavity, the peritoneum showed Multiple, grayish -Clinical signs were recorded as loss of appetite, white nodules of varying size (1 to 2cm diameter) were rapid weight loss, occasional vomiting, dehydration, scattered throughout surfaces of the peritoneum.(Fig. pale mucus membrane, rough and loose skin and -2).Such nodules also recorded on surface and deep in ruffled hair coat, extreme rapid emaciation and with to parenchyma of the liver, lungs, spleen, intestine and distended abdomen (Fig.-1).The tiger was free from mesenteric lymph node (Fig.-2,3,4).The liver was internal and external parasitic infestation.Blood severely congested and swollen.Tracheobronchial smear examination was negative for blood protozoa.and mediastinal lymph nodes were enlarged with Clinical examination revealed, normal body temperature neoplastic growths.

Figure- 1 .
Figure-1.Weak emaciated carcass with distended abdomen of female white tiger Figure-2.Mesothelioma: Peritoneum: variable size multiple grayish -white nodules Figure-3.Tumour Nodules on theserosal surface and deep in to parenchyma of the liver

Figure- 5 .Figure- 6 .
Figure-5.Mesothelioma peritoneummultiple layers of neoplastic mesothelial cells with well supported by collagenous connective tissues.(H &E 40X) Figure-6.Mesothelioma hepatic metastasislarger vesicular nuclei and prominent nucleoli and were arranged in cord and nest types.(H &E 40X) Methods of the Armed Forces institute of Pathology.recorded the distance metastasis of the tumour cells III edition, McGraw Hill Book Company, London.which contradicts with the findings.The mesothelial 5. Pazhanivel, N., Balchandran, C., Vairamuthu, S., cells have granular cytoplasm, larger vesicular Hemalatha, S. and Murali Manohar, B. (2007).A rare nucleus with prominent nucleolus and the neoplastic case of multiple organ metastatic mesothelioma in a cells of different organs were nest types of dog.Indian J. Vet.Pathol.31(2): 169 -171.