Aim: To test the sensitivity of S. Enteritidis for selected antibiotics.
Materials and Methods: S. Enteritidis isolates obtained from different
samples of chicken, mutton, turkey meat, faecal and cloacal samples of poultry
and turkey, eggs, water and feed were subjected for sensitivity and resistance
to selected antibiotics like- Chloramphenicol (30µg), Gentamicin (10 µg),
Nalidixic Acid (30 µg), Tetracycline (30 µg), Ciprofloxacin (5 µg), Amikacin (30
µg), Amoxicillin (25 µg), Ampicillin (10 µg), Streptomycin (10 µg) and
Sulfonamide (30 µg). Antimicrobial susceptibility of the isolates was
established by the disk diffusion assay with MH (Muller-Hinton) agar in
accordance with French National Antibiogram Committee Guidelines.
Results:
The sensitivity of S. Enteritidis was 100% for ciprofloxacin followed by
chloramphenicol and amikacin (96%), gentamycin (90%), amoxicillin (82%),
streptomycin (80%), tetracycline (76%), nalidixic acid (68%), ampicillin (58%)
and sulfonamide (10%). The resistance was highest for sulfonamide (76%) followed
by ampicillin (32%), nalidixic acid (30%) and 6-20% for gentamycin, amoxicillin
and tetracycline.
Conclusion: S. Enteritidis isolates were more sensitive
to ciprofloxacin, chloramphenicol, amikacin, gentamycin, streptomycin,
amoxicillin and tetracyclines and less sensitive to sulfonamides. Higher
resistance was observed with sulfonamide followed by ampicillin and nalidixic
acid.
Keywords: antibiotic resistance, antibiotic sensitivity, S.
Enteritidis
