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3. Isolation and
identification of Staphylococcus aureus
from milk and milk products and their drug
resistance patterns in Anand, Gujarat -
H. C. Thaker, M. N. Brahmbhatt and J. B. Nayak
Vet World. 2013; 6(1): 10-13
Aim: The study was carried out with aim to
isolate Staphylococcus aureus from milk and milk
products (pedha and curd) and determine
antibiogram pattern of S. aureus isolates.
Materials and Methods: During 9 months
duration of study a total of 160 milk and milk
product samples (pedha and curd) were collected
from different places in and around Anand city
such as milk collection centre of Co-operative
milk dairies, cattle farms, individual household,
milk vendors and sweet shops. The samples were
collected under aseptic precautions and were
enriched in Peptone Water (PW) followed by direct
plating on selective media viz. Baird-Parker Agar.
The presumptive S. aureus isolates were identified
by biochemical tests. Antibiogram pattern of S.
aureus to antimicrobial agents were evaluated by
disk diffusion method.
Results: Analysis of
result revealed that out of total 160 samples of
milk (100) and milk products i.e. curd (30) and
pedha (30) resulted in the isolation of 10
isolates (6.25%) of S. aureus. In the present
study S. aureus isolates were found variably
resistant to the antibiotics tested. The S. aureus
isolates showed highest sensitivity towards
cephalothin (100.00%), co-trimoxazole (100.00%),
cephalexin (100.00%) and methicillin (100.00%)
followed by gentamicin (90.00%), ciprofloxacin
(80.00%), oxacillin (70.00%), streptomycin
(60.00%) and ampicillin (60.00%). The pattern
clearly indicated that the overall high percent of
S. aureus isolates were resistant to Penicillin-G
(100.00%) followed by ampicillin (40.00%),
oxytetracycline and oxacillin (20.00%) and
streptomycin and gentamicin (10.00%).
Conclusions: Results clearly suggested a
possibility of potential public health threat of
S. aureus resulting from contamination of milk and
milk products with pathogenic bacteria is mainly
due to unhygienic processing, handling and
antibiogram pattern, milk products, S. aureus,
staphylococcal food poisoning
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