and blood biochemical parameters of Jaffarabadi heifers

Aim: The objective of an experiment was conducted to evaluate the effect of feeding bypass Methonine-Lysine on hematological and biochemical parameters on growing Jaffrabadi heifers of age from 12 to 18 month. Material and Methods: Eighteen Jaffrabadi heifers were divided into three groups of equal number: control group (T ), 1 st cotton seed cake (CSC) (T ) and bypass Methionine-Lysine supplementation (T ). Blood samples were collected on the 1 and 2 3 rd 63 day of the trail. A close examination of the nutritive effects of treatment of CSC and bypass Methionine and Lysine (M-L) supplementation on hematological and biochemical values in the blood (white blood cell count (WBC), red blood cell count (RBC), hemoglobin (Hb), pack cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelets count (PLT), total protein, albumin, globulin, blood urea nitrogen (BUN), creatinine and creatine kinase was carried out. Results: BUN had a tendency to decrease in T and T (P<0.05) by the end of the trail with compare to control but no significant 2 3 difference was observed between T and T . No significant differences were found between treatments in other hematological 2 3 and biochemical parameters like total protein, albumin, globulin, creatinine and creatine kinase. Conclusion: Bypass Methionine and Lysine have no effect on hematological and biochemical parameters except blood urea nitrogen (BUN) in growing Jaffrabadi heifers.


Introduction
Lysine and Histidine might be the limiting amino acids in growing beef steers [4].Archibeque et al., [5] Ruminant animals require two types of digestible identified Methionine as a limiting amino acid for protein.The first is a degradable protein in the rumen growing cattle.Supplement of rumen-protected Methionine which is degraded by the micro-organisms in rumen to improves the average daily gain of growing cattle [6].produce microbial protein.The second is the bypass Blood metabolites and hematological indices of beef protein which escapes microbial digestion in rumen cows can be affected by feeding rumen protected and is digested directly in the small intestine.Rumen Methionine during gestation [7].degradable protein is used to synthesize microbial Due to limited referential data, the effect of bypass protein which is valuable metabolisable protein source Methionine and Lysine, on Jaffrabadi buffaloes, as one for the animal.Metabolisable protein (MP) consists of of the potentially deficient amino acids in nutrition of bypass protein and microbial protein.Excessive growing heifers, has been studied in this experiment.degradation of valuable protein in rumen by the microorganisms results in degradation of high quality protein

and up gradation of poor quality protein as well as in
The study was approved by the committee formed some losses of Nitrogen as urea in urine.Rumen for the research by the university authority.Eighteen microbes have the ability to synthesize all the essential Jaffrabadi buffalo heifers of age from 12 to 18 month amino acids but microbial population in rumen alone were taken in experiment at Cattle Breeding Farm, cannot supply all amino acids to a fast growing dairy Junagadh Agricultural University, Junagadh.These animal in required amount, and the ruminally animals were divided into three group of six each.T 1 undegradable protein/ruminally degradable protein feed with 2 Kg Groundnut Gottar + Concentrate mixture system began to get more usage [1].Ruminally to meet out DCP requirement, T feed with 2 Kg undegraded feed protein from vegetable sources are 2 Groundnut Gottar + Concentrate mixture and cotton usually low in content of Methionine or Lysine [2].
seed cake (CSC) (50:50) to meet out DCP requirement Methionine and Lysine were reported as the first and and T feed with in addition to T feeding rumen bypass second respectively limiting amino acids for synthesis 3 1 of tissue protein in growing ruminants [3].Methionine, Methionine and Lysine was supplied at @ 5 and 10 g / head/day respectively with mature pasture grass hay ad of different doses of blood meal rich in Methionine and lib to each group.DCP values of each constituent of Lysine there were decrease of TEC, Hb concentration feed were analysed and mention in Table -1.Blood and PCV and increase of erythrocyte sedimentation samples were drawn by puncture of jugular vein in rate (ESR) content in the birds.It may be due to higher sterile vial on initial (day 1) and final stage (day 63) of growth rate of birds compare to control group.Donkoh experiment for blood analysis.Hematological parameters et al. [11] and Odunsi et al. [12] reported that hematowere analyzed by automatic hematology analyzer (BC-logical parameters are unchanged in protein treatment Vet 2800) and blood chemistry was analyzed from in birds.plasma for plasma total proteins by Biuret method, Blood constituents in broilers were not significantly creatinine by modified Jaffe's method, creatinine increased by different levels of Methionine.Hemoglokinase by UV-kinetic method and BUN by NED-DYE bin, hematocrit and red blood cells were not significantly method.Body weights of experimental animals were affected by dietary Methionine.MCV and MCH were taken at initiation and termination of experiment and increased significantly by increasing Methionine level, their difference were shown in Table-2.

Experimental data was analyzed statistically
Blood biochemical data: No significant difference was followed by Complete Randomized Design (CRD) as observed in biochemical parameters like Total protein, per Snedecor and Cochran [8].Treatment differences albumin, globulin, creatinine and creatine kinase were considered significant for P<0.05.
except BUN, which is showing lower significant

Result and Discussion
(P<0.05) difference between the treatment.Liker et al. [9] showed decrease plasma urea Table 3 and 4 are showing data of effect of different concentration with treatment of rumen-protected treatments verses control group of hematology and Methionine at trial of day 94 but no significant blood biochemical parameters in Jaffrabadi heifers differences were found between the groups in total respectively.protein, albumin and creatinine.Plasma total protein Hematological data: No significant difference was and globulin were not significantly affected by Lysine.observed in counts of WBC, RBC, PLT, PCV, hemo-However, albumin was increased with increasing globin and other indices of blood parameters, but mean Lysine [13].Similar significant decreasing trend of values were observed increase in WBC, RBC, BUN was observed in dairy cow after providing hemoglobin, PCV and PLT between control group and palletize Methionine-Lysine supplementation by Ye et CSC and M-L supplementation ration groups.

al. [14]. Similar non-significant finding was observed for
In dairy cows, urea nitrogen in blood, urine, and hematological parameters viz WBC, RBC, Hb, PCV, milk is mainly derived from excessive ammonia PLT, MCH and MCV by Liker et al. [9] after feeding absorbed through the rumen wall from degradation of rumen-protected Methionine to cattle.Shahidullah et dietary CP in the rumen and from the deamination of al. [10]  This in-vivo study on Jaffrabadi heifers demon-Lysine and CSC significantly decreased urea N strated the potential for supplementing Methionine and Lysine without much economic impact for improvement concentration in plasma with the control.The decrease in digestibility of nutrients, intake of DCP and positive in urea nitrogen concentration indicates that nitrogen balance without any effect on the health of heifers.supplementation of protected Methionine and Lysine to the control diet in the present study may have Author's contribution improved AA balance in MP and resulted in less All authors contributed equally.All authors read and deamination of absorbed AA.This consideration was approved the final manuscript.further supported by the fact that in the present study, treatment M-L had higher nitrogen utilization

Table - 1
. Percent average composition of feed and fodder (on DM basis) fed to experimental heifers Effect of methionine and lysine supplementation on body weight of Jaffrabadi heifers (n=6) observed blood profile studies after treatment amino acids (AA), which may be from MP and

Table - 3
. Effect of methionine and lysine supplementation on hematological parameters of Jaffrabadi heifers (n=6)

Table - 4
. Effect of methionine and lysine supplementation on blood biochemistry of Jaffrabadi heifers (n=6) NS -Non-significant, SEM-Standard error mean, CD-Critical difference, CV-Coefficient of variance