Veterinary World


ISSN (Online): 2231-0916


 Editorial board

 Instructions for authors

 Reviewer guideline

 Open access policy




Open Access

Copyright: The authors. This article is an open access article licensed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited.

Research (Published online : 29-10-2013)

19. Detection and differentiation of sheeppox virus and goatpox virus from clinical samples using 30 kDa RNA polymerase subunit (RPO30) gene based PCR - R. Santhamani, R. Yogisharadhya, G. Venkatesan, S. B. Shivachandra, A. B. Pandey and M. A. Ramakrishnan
Veterinary World, 6(11): 923-925


doi: 10.14202/vetworld.2013.923-925


1. Babiuk, S., Bowden, T. R., Boyle, D.B., Wallace, D. B., Kitching, R. P. (2008) Capripoxviruses: an emerging world wide threat to sheep, goats a.nd cattle. Transbound. Emerg. Dis. 55: 263–272.
2. International Office of Epizootics. Biological Standards Commission, International Office of Epizootics. Inter- national Committee (2008) Manual of diagnostic tests and vaccines for terrestrial animals?: mammals, birds, and bees. Office international des épizooties, Paris.
3. Carn, V. M. (1993) Control of capripoxvirus infections. Vaccine 11:1275–1279.
4. Bhanuprakash, V., Indrani, B.K., Hosamani, M. and Singh, R.K. (2006) The current status of sheep pox disease. Comp. Immunol. Microbiol. Infect. Dis. 29:27–60.
5. Mondal, B., Hosamani, M., Dutta, T.K., Senthilkumar, V.S., Rathore, R. and Singh, R.K. (2004) An outbreak of sheep pox on a sheep breeding farm in Jammu, India. Sci. Tech. Rev. 23: 943–949.
6. Verma, S., Verma, L.K., Gupta, V.K., Katoch, V.C., Dogra, V., Pal, B. and Sharma, M. (2011) Emerging Capripoxvirus disease outbreaks in Himachal Pradesh, a northern state of India. Transbound. Emerg. Dis. 58:79–85.
7. Bhanuprakash, V., Venkatesan, G., Balamurugan, V., Hosamani, M., Yogisharadhya, R., Chauhan, R.S., Pande, A., Mondal, B. and Singh, R.K. (2010) Pox outbreaks in sheep and goats at Makhdoom (Uttar Pradesh), India: evidence of sheeppox virus infection in goats. Transbound. Emerg. Dis. 57: 375–382.
8. Hosamani, M., Nandi, S., Mondal, B., Singh, R.K., Rasool, T.J. and Bandyopadhyay, S.K. (2004) A Vero cell-attenuated Goatpox virus provides protection against virulent virus challenge. Acta Virol 48:15–21.
9. Yogisharadhya, R., Bhanuprakash, V., Hosamani, M., Venkatesan, G., Balamurugan, V., Bora, D.P., Bhanot, V., Prabhu, M. and Singh, R.K. (2011) Comparative efficacy of live replicating sheeppox vaccine strains in Ovines. Biologicals 39:417–423.
10. Balinsky, C.A, Delhon, G., Smoliga, G., Prarat, M., French, R.A., Geary, S.J., Rock, D.L. and Rodriguez, L.L. (2008) Rapid preclinical detection of sheeppox virus by a real-time PCR assay. J. Clin. Microbiol. 46:438–442.
PMid:18032617 PMCid:PMC2238129
11. Hosamani, M., Mondal, B., Tembhurne, P.A., Bandyo- padhyay, S.K., Singh, R.K. and Rasool, T.J. (2004) Differen- tiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene. Virus Genes 29:73–80.
12. Lamien, C.E., Le Goff, C., Silber, R. (2011) Use of the Capripoxvirus homologue of Vaccinia virus 30 kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target: development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus. Vet. Microbiol. 149:30–39.
13. Lamien, C.E., Lelenta, M., Goger, W., Silber, R., Tuppurainen, E., Matijevic, M., Luckins, A.G. and Diallo, A. (2011) Real time PCR method for simultaneous detection, quantitation and differentiation of capripoxviruses. J. Virol. Meth. 171: 134- 140.
14. Yousif, A.A., Al-Naeem, A.A., Al-Ali, M.A. (2010) Rapid non-enzymatic extraction method for isolating PCR-quality camelpox virus DNA from skin. J. Virol. Meth. 169: 138-142.
15. Beard, P.M., Sugar, S., Bazarragchaa, E., Gerelmaa, U., Tserendorj, S., Tuppurainen, E. and Sodnomdarjaa, R. (2010) A description of two outbreaks of capripoxvirus disease in Mongolia. Vet. Microbiol. 142:427–431.
PMid:19939588 PMCid:PMC2866252
16. Ireland, D.C. and Binepal, Y.S. (1998) Improved detection of capripoxvirus in biopsy samples by PCR. J. Virol. Meth. 74:1–7.
17. Yan, X-M., Chu, Y-F., Wu, G-H., Zhao, Z-X., Li, J., Zhu, H- X. and Zhang, Q. (2012) An outbreak of sheep pox associated with goat poxvirus in Gansu province of China. Vet. Microbiol. 156:425–428.
18. Heine, H.G., Stevens, M.P., Foord, A.J. and Boyle, D.B. (1999) A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. J. Immunol. Meth. 227:187–196.
19. Mangana-Vougiouka, O., Markoulatos, P., Koptopoulos, G., Nomikou, K., Bakandritsos, N. and Papadopoulos, P. (2000) Sheep poxvirus identification from clinical specimens by PCR, cell culture, immunofluorescence and agar gel immunoprecipitation assay. Mol. Cell. Probes 14:305–310.
20. Orlova, E.S., Shcherbakova, A.V., Diev, V.I. and Zakharov, V.M. (2006) Differentiation of capripoxvirus species and strains by polymerase chain reaction. Mol. Biol. (Mosk) 40: 158–164.
21. Venkatesan, G., Balamurugan, V., Yogisharadhya, R., Kumar, A. and Bhanuprakash, V. (2012) Differentiation of sheeppox and goatpox viruses by polymerase Chain reaction-restriction fragment length polymorphism. Virol. Sin. 27:352–358.