|
|
Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research (Published
online : 14-08-2013)
11. Genetic characterization of Andaman Desi pig, an
indigenous pig germplasm of Andaman and Nicobar group of islands, India by
microsatellite markers - Arun Kumar De, A. Kundu, M.S. Kundu, Jai
Sunder, S. Jeyakumar and M. Ramachandran
Veterinary World, 6(10): 750-753
doi:
10.14202/vetworld.2013.750-753
Abstract
Aim:
The present study was carried out to characterize Andaman Desi
pig genetically using 23 FAO recommended microsatellite markers.
Materials and Methods: 25 blood samples were collected from
genetically unrelated Andaman Desi pigs and DNA was isolated by
standard procedure of phenol/chloroform. The genomic DNA was
amplified by polymerase chain reaction (PCR) at 23 microsatellite
loci and the PCR products were resolved by denaturing urea
polyacrylamide gel electrophoresis and alleles were visualized
after silver nitrate staining. The data were analyzed for allele
size range, number of alleles, allelic frequencies, heterozygosity
and polymorphism information content (PIC) for each locus.
Results: The allele size range varied from 86–116 bp at locus
SW936 to 280–296 at locus IGFI. The total number of alleles ranged
between 5 (S0228, SW122, SW951, SW24 and S0178) and 12 (S0355).
The effective number of alleles ranged from 3.14 (SW24) to 8.1
(S0355). The mean observed and expected heterozygosities were
0.69±0.01 and 0.77±0.01 respectively. The mean PIC for all the 23
studied loci was 0.74±0.01.
Conclusions: The Andaman Desi pig was characterized at genetic
level and it was found that the genetic diversity of this
indigenous breed was high.
Keywords: Andaman Desi pig, genetic characterization,
microsatellite, polymorphic information content
References
|
1. Kundu, A., Sunder, J., Jeyakumar, S., Verma, S. K., Kundu,
M. S., De, A. K. and Srivastava, R. C. (2010) Livestock and
poultry production policy for Andaman and Nicobar Islands: a
scientific perspective. Published by Director, CARI, Port
Blair. pp 1-48. |
|
|
2. Zanga, J., Chimonyo, M., Kanengoni, A., Dzama, K. and
Mukaratirwa, S. (2003) A comparison of the susceptibility of
growing Mukota and Large White pig genotypes to infection with
Ascaris suum. Vet. Res. Comm. 27: 653–660.
http://dx.doi.org/10.1023/A:1027320428646
PMid:14672454 |
|
|
|
3. Mashatise, E., Hamudikuwanda, H., Dzama, K., Chimonyo, M.
and Kanengoni, A. (2005) Socio-economic roles, traditional
management systems and reproductive patterns of Mukota pigs in
semi-arid north-eastern Zimbabwe. Bunda Journal of
Agriculture, Environmental Science and Technology 3: 97–105. |
|
|
4. Kumaresan, A., Bujarbaruah, K. M., Pathak, K. A., Chhetri,
B, Das, S. K., Das, A. and Ahmed, S. K. (2007) Performance of
pigs reared under traditional tribal low input production
system and chemical composition of nonconventional tropical
plants used as pig feed. Livest. Sci. 107: 294–298.
http://dx.doi.org/10.1016/j.livsci.2006.12.007 |
|
|
5. Behl, R., Sheoran, N., Behl, J. and Vijh, R. K. (2006)
Genetic analysis of Ankamali pigs of India using
microsatellite markers and their comparison with other
domesticated Indian pig types. J. Anim. Breed. Genet. 123:
131-135.
http://dx.doi.org/10.1111/j.1439-0388.2006.00568.x
PMid:16533367 |
|
|
6. Scali, M., Vignani, R., Bigliazzi, J., Paolucci, E.,
Bernini, A., Spiga, O., Niccolai, N. and Cresti, M. (2012)
Genetic differentiation between Cinta Senese and commercial
pig breeds using microsatellite. Electron. J. Biotechnol.
15(2): 1-11.
http://dx.doi.org/10.2225/vol15-issue2-fulltext-1 |
|
|
7. Fredholm, M., Wintero, A. K., Christensen, K., Christensen,
B., Nielson, P. B., Davies, W. and Archibald, A. (1993)
Characterization of 24 porcine (dA-dC)n–(dT-dG)n
microsatellites: genotyping of unrelated animals from four
breeds and linkage studies. Mamm. Genome 4: 187–192.
http://dx.doi.org/10.1007/BF00417561
PMid:8499651 |
|
|
8. van Zeveran, A., Peelman, L., van de Weghe, A. and Bouquet,
Y. (1995) A genetic study of four Belgian pig populations by
means of seven microsatellite loci. J. Anim. Breed. Genet.
112: 191-204.
http://dx.doi.org/10.1111/j.1439-0388.1995.tb00558.x |
|
|
9. Laval, G., Iannucelli, C., Legault, C., Milan, D., Gronen,
M. A. M., Giuffra, E., Anderson, L., Nissen, P. M., Jorgenson,
C. B., Beckman, P., Geldermann, H., Foulley, J. L., Chevalet,
C. and Ollivier, L. (2000) Genetic diversity in eleven
European pig breeds. Genet. Sel. Evol. 32: 187–203.
http://dx.doi.org/10.1186/1297-9686-32-2-187
PMid:14736401 PMCid:PMC2706869 |
|
|
10. Lemus-Flores, C., Ulloa-Arvizu, R., Ramos-Kuri, M.,
Estrada, F. J. and Alonro, R. A. (2001) Genetic analysis of
Mexican hairless pig population. J. Anim. Sci. 79: 3021– 3026.
PMid:11811455 |
|
|
11. Fabuel, E., Barragan, C., Silio, L., Rodriguez, M. C. and
Toro, M. A. (2004) Analysis of genetic diversity and
conservation priorities in Iberian pigs based on
microsatellite markers. Heredity 93: 104–113.
http://dx.doi.org/10.1038/sj.hdy.6800488
PMid:15150539 |
|
|
12. Fang, M., Hu, X., Jiang, T., Braunsweig, M., Hu, L., Du,
Z., Feng, J., Zhang, Q., Wu, C. and Li, N. (2005) The
phylogeny of Chinese indigenous pig breeds inferred from
microsatellite markers. Anim. Genet. 36: 7–13.
http://dx.doi.org/10.1111/j.1365-2052.2004.01234.x
PMid:15670125 |
|
|
13. Vicente, A. A., Carolino, M. I., Sousa, M. C. O., Ginja,
C., Silva, F. S., Martinez, A. M., Vega-Pla, J. L., Carolino,
N. and Gama, L. T. (2008) Genetic diversity in native and
commercial breeds of pigs in Portugal assessed by
microsatellites. J. Anim. Sci. 86: 2496-2507.
http://dx.doi.org/10.2527/jas.2007-0691
PMid:18567729 |
|
|
14. Sollero, B. P., Paiva, S. R., Faria, D. A., Guimaraes, S.
E. F., Castro, S. T. R., Egito, A. A., Albuquerque, M. S. M.,
Piovezan, U., Bertani, G. R. and Mariante, A. D. (2009)
Genetic diversity of Brazilian pig breeds evidenced by
microsatellite markers. Livest. Sci. 123: 8-15.
http://dx.doi.org/10.1016/j.livsci.2008.09.025 |
|
|
15. Chang, W. H., Chu, H. P., Jiang, Y. N., Li, S. H., Wang,
Y., Chen, C. H., Chen, K. J., Lin, C. Y. and Ju, Y.T. (2009)
Genetic variation and phylogenetics of Lanyu and exotic pig
breeds in Taiwan analyzed by nineteen microsatellite markers.
J. Anim. Sci. 87: 1-8.
http://dx.doi.org/10.2527/jas.2007-0562
PMid:18708610 |
|
|
16. Fang, M., Hu, X., Jin, W., Li, N. and Wu, C. (2009)
Genetic uniqueness of Chinese village pig populations inferred
from microsatellite markers. J. Anim. Sci. 87: 3445-3450.
http://dx.doi.org/10.2527/jas.2008-1632
PMid:19648495 |
|
|
17. Kaul, R., Singh, A., Vijh, R. K., Tantia, M. S. and Behl,
R. (2001) Evaluation of the genetic variability of 13
microsatellite markers in native Indian pigs. J. Genet. 80(3):
149-53.
http://dx.doi.org/10.1007/BF02717911
PMid:11988634 |
|
|
|
18. Yeh F. C., Yang, R. and Boyle, T. (1999) POPGENE Version
1.31, Microsoft Window Based Freeware for Population Genetic
Analysis. University of Alberta and Centre for International
Forestry Research. Available at: http://www.ualberta.ca/~fyeh/
Accessed on 12-06-2013. |
|
|
19. Botstein, D., White, R. L., Skolnick, M. and Davis, R.L.
(1980) Construction of genetic linkage map in man using
restriction fragment length polymorphisms. Am. J. Hum. Genet.
32: 314–331.
PMid:6247908 PMCid:PMC1686077 |
|
|
20. Amaral, A. J., Ferretti, L., Megens, H. J., Crooijmans, R.
P. M. A., Nie, H., Ramos-Onsins, S. E., Perez-Enciso, M.,
Schook, L. B. and Groenen M. A. M. (2011) Genome-wide
footprints of pig domestication and selection revealed through
massive parallel sequencing of pooled DNA. PLoS ONE 6 (4): p.
e14782.
http://dx.doi.org/10.1371/journal.pone.0014782
PMid:21483733 PMCid:PMC3070695 |
|
|
21. Amigues, Y., Boitard, S., Bertrand, C., Sancristobal, M.
and Rocha, D. (2011) Genetic characterization of the Blonde
d'Aquitaine cattle breed using microsatellite markers and
relationship with three other French cattle populations. J.
Anim. Breed. Genet. 128(3): 201-8.
http://dx.doi.org/10.1111/j.1439-0388.2010.00890.x
PMid:21554414 |
|
|
22. Tamara, A. J., Choumane, W. and Hmeshe, M. (2012)
Characterization and Estimation of Genetic Diversity in Two
Syrian Chicken Phenotypes Using Molecular Markers. Int. J.
Poult. Sci. 11 (1): 16-22, 2012.
http://dx.doi.org/10.3923/ijps.2012.16.22 |
|
|
|
23. Nidup, K. and Moran, C. (2011) Genetic diversity of
domestic pigs as revealed by microsatellites: a mini review.
Genomics and Quantitative Genetics 2: 5-18. |
|
|
24. Sollero, B. P., Paiva, S. R., Faria, D. A., Guimarăes, S.
E. F., Castro, S. T. R., Egito, A. A., Albuquerque, M. S. M.,
Piovezan, U., Bertani, G. R. and da S. Mariante, A. (2009)
Genetic diversity of Brazilian pig breeds evidenced by
microsatellite markers. Livest. Sci. 123 (1): 8-15.
http://dx.doi.org/10.1016/j.livsci.2008.09.025 |
|
|
25. Swart, H., Kotze, A., Olivier, P. A. S. and Grobler, J. P.
(2010) Microsatellite-based characterization of Southern
African domestic pigs (Sus scrofa domestica). S. Afr. J. Anim.
Sci. 40 (2): 121-132.
http://dx.doi.org/10.4314/sajas.v40i2.57280 |
|
|
26. Chen, Y. C., Hsu, J. T., Chien, C. C., Leu, Y. C., Chyr,
C. Y. L., Lin, D. Y., Lin, E.-C., Chen, C. H. and Wang, P. H.
(2012) Investigation of genetic relationships among Taiwan
Black Pigs and other pig breeds in Taiwan based on
microsatellite markers. Anim. Biotechnol. 23 (4): 278-90.
http://dx.doi.org/10.1080/10495398.2012.700667
PMid:23134307 |
|
|
