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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online : 12-07-2013)
17.
Biochemical, histopathological and ultra structural profile after pulsed water
medication of enrofloxacin in broiler chickens
-
Veerapandian Sureshkumar, Ghadevaru Sarathchandra and Jayaramachandran Ramesh
Vet World. 2013; 6(9):
668-673
doi:
10.14202/vetworld.2013.668-673
Abstract
Aim:
A pharmacological study was undertaken to evaluate the safety
and adverse effects of enrofloxacin administration in broiler
chickens by assessing the serum biochemical parameters, associated
histopathological and ultra structural changes in liver and
kidney.
Materials and Methods: Birds in the treatment group were
administered with enrofloxacin at the recommended therapeutic dose
10mg/kg body weight via drinking water for five successive days,
while the control group (untreated group) received non medicated
water. Serum biochemical parameters viz., total protein, albumin,
lactate dehydrogenase, alkaline phosphatase, creatine kinase,
lipase, triglyceride, gamma glutamyl transferase, urea,
uric acid and creatinine were estimated at 24hour and 48hour
intervals during the dosing and withdrawal periods, respectively.
Liver and kidney tissue samples collected from 1, 3, 5, 7, and 9
days post treatment groups were subjected to histopathological and
ultrastructural examinations.
Results: There was no significant change (p>0.05) in total
protein, albumin, lactate dehydrogenase, alkaline phosphatase,
creatine kinase, lipase, triglyceride and urea levels in the
enrofloxacin administered broiler chickens at all the time points
evaluated. However, a significant increase (p<0.05) in gamma
glutamyl transferase, uric acid and creatinine levels were
observed after the 4th
dose of the enrofloxacin and on day 1 post
treatment. During the withdrawal period, the elevated levels
declined gradually and showed the trend towards control values as
evidenced by a statistically insignificant difference on 3, 5, 7
and 9 days post treatment when compared to that of control group.
These biochemical changes were substantiated by histopathological
and ultrastructural changes elicited in liver and kidney.
Conclusion: The reversible trend observed in serum biochemical
parameters, histopathological and ultra structural alterations in
liver and kidney during the withdrawal period suggests that
enrofloxacin is safe if administered to broiler chickens at the
recommended therapeutic dose and if the stipulated withdrawal
period is strictly adhered to.
Keywords: biochemical parameters, broiler chickens,
enrofloxacin, histopathology, ultrastructural, veterinary
pharmacovigilance
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