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Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Research
(Published
online: 06-01-2014)
2. Studies on acute toxicity of synthetic pyrethroid λ-cyhalothrin
on freshwater fish Labeo rohita -
P. D. Gadhave, R. S. Brar, H. S. Banga and A. Dhawan
Veterinary World, 7(1): 7-9
doi:
10.14202/vetworld.2014.7-9
Abstract
Aim: To study the acute
toxicity of λ-cyhalothrin in freshwater fish Labeo rohita by
determining the 96 hours lethal concentration 50 (96 hrs LC50) and
behavioural patterns of exposed fish.
Materials and Methods: A total 270 fingerling size fish
were used to determine the 96 hours lethal concentration 50 (96
hrs LC50) of λ-cyhalothrin as per the guideline of Organization
for Economic Co-operation and Development (OECD) 203 except the
loading density which was higher than the recommended density 1.0
g/l to reduce the chemical loss. The fingerlings were brought from
the local fish hatchery, disinfected and acclimatized for 14 days
in laboratory conditions. 60 fingerlings, 10 each were divided
into six plastic tubs of 35 L capacity with 0 ug/L (control), 10
ug/L ethanol only (vehicle control), 0.1 ug/L, 1ug/L, 5 ug/L and
10 ug/L of λ-cyhalothrin in 20L of dechlorinated tap water. Static
renewable bioassay was conducted using five selected doses in
between concentrations causing 10 to 100% mortality i.e. 2 ug/L,
2.5 ug/L, 3 ug/L, 3.5 ug/L and 4 ug/L along with control and 4.0
ug/L of acetone only as vehicle control. Water quality parameters
like temperature, pH, total hardness and total alkalinity were
measured daily. The experiment was repeated three times.
Behavioural pattern and mortality was recorded periodically.
Results: The fish exposed to chemical showed erratic
swimming, loss of balance, surfacing and convulsions in a dose
dependent manner. 2.72 ug/L was calculated as 96 hrs LC50 value
for λ-cyhalothrin in Labeo rohita.
Conclusions: λ-cyhalothrin was reported as highly toxic
chemical for Labeo rohita producing altered behavioural pattern in
dose dependent manner.
Keywords: λ-cyhalothrin, fingerlings, Labeo rohita
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