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Research (Published online: 24-01-2014)

8. Detection and differentiation of infectious bursal disease virus from the outbreaks in two layer farms by PCR-RFLP in Jos, Nigeria - P. D. Luka, B. Yakubu, A. R. Jambol, B. J. Audu, B. B. Dogonyaro and O. A. Owolodun
Veterinary World, 7(1): 30-33


doi: 10.14202/vetworld.2014.30-33


1. Long, F.Y., Guo, Y. M., Wang, Z., Liu, D., Zhang, B.K., and Yang, X. (2011) Conjugated linoleic acids alleviate infectious bursal disease virus-induced immunosuppression in broiler chickens. Poultry Science. 90 :1926–1933.
2. Delmas, B., Mundt, E.,Vakharia, V.N., and Wu, J.L. (2011) Family birnaviridae. In: King, A.M.Q., Lefkowitz, E., Adams, M.J., Carstens, E.B. (Eds) Virus Toxonomy Ninth Report of the International Committee on Taxonomy of Viruses. Academic press Inc., San Diego, California.507.
3. Arnold, M., Durairaj, V., Mundt, E., Schulze, K., Breunig, K.D., and Behrens, S.E. (2012) Protective Vaccination against Infectious Bursal Disease Virus with Whole Recombinant Kluyveromyces lactis Yeast Expressing the Viral VP2 Subunit. PLoS PLoS ONE, 7(9) : e428-70.
4. Wang, Y., Wu, X., Li, H., Wu, Y., Shi, L., Zheng, X., Luo, M., Yan, Y., and Zhou, J. (2009) Antibody to VP4 protein is an indicator discriminating pathogenic and nonpathogenic IBDV infection. Mol. Immunol. 46 (10):1964-9.
5. Abdel-Alem, G.A., Awaad, M.H.H., and Saif, Y.M. (2003) Characterization of Egyptian field strains of infectious bursal disease virus. Avian Dis. 47: 1452-1457.
6. Van Den Berg, T.P (2000) Acute infectious bursal disease in poultry: A review. Avian Pathology, 29 (3): 175-194.
7. Jackwood, J.D., Sommer-Wagner, S.E., Stoute, S.T., Woolcock, P.R., Crossley, B.M., Hietala, S.K., and Charlton, B.R. (2009) Characteristics of a Very Virulent Infectious Bursal Disease Virus from California. Avian Diseases, 53(4): 592-600.
8. Escaffre, O., Nouen, C.L., Amelot, M., Ambroggio, X., Ogden, M.K., Guionie, O., Toquin, D., Muller, H., Islam, M., and Eterradossi, N. (2013) Both genome segments contribute to the pathogenicity of very virulent infectious bursal disease virus. J. Virol. 87(5): 2767-2780.
9. Office International for Epizootics (OIE): Infectious bursal disease In Manual of Standards for Diagnostic Tests and Vaccines for Terrestrial Animals. 5 edition. Paris, OIE; Accessed on 10 July 2013.
10. Islam, M.R., Rahman, S., Noor, M., Chowdhury, E.H., and Müller, H. (2012) Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis. Arch Virol. 157(2):333-6.
11. Zahoor, A.M., Abubakar, M., Naim, S., Khan, Q.M., and Arshed, M.J. (2011) Molecular Typing of Field Isolates from two outbreaks of Infectious Bursal Disease Virus from Pakistan. Vet World, 4(7): 297-300.
12. Ojo, M.O., Oduye, O.O., Noibi, L.M., and Idowu, A.L. (1973) Gumboro-like disease in Nigeria. Trop. Anim Hlth and Prod. 5: 52-56.
13. Hassan, M.K. (2004) Very virulent infectious bursal disease virus in Egypt: Epidemiology, isolation and immunogenicity of classic vaccine. Vet. Res. Comm, 28: 347-356.
14. Jackwood, D.J., and Sommer, S.E. (1999) Restriction fragment polymorphisms in the VP2 gene of infectious bursal disease viruses from outside the United States. Avian Diseases, 43: 310–314.
15. Ezeibe, M.C.O., Okoye, J.O.A., Ogunniran, T.M., Animoke, P.C.J., Nwankwo, I.A., and Ngene, A.A. (2013) Mortality rates from a Nigerian isolate of the Infectious Bursa Disease Virus and passive haemagglutination antibody titer that protects chicks against challenge with the virus isolate. Health, 5: 1355-1359.
16. Brown, M.D., Green, P., and Skinner, M.A. (1994) VP2 sequences of "very virulent" isolates of infectious bursal disease virus are closely related to each other but are distinct from those of "classical" strains. J. Gen. Virol. 75: 675-680.
17. Kataria, R.S., Tiwari, A.K., Butchaiah, G., and Kataria, J.M. (1999) Differentiation of infectious bursal disease virus strains by restriction analysis of RT-PCR-amplified VP2 gene sequences. Acta Virol. 43: 245-249.
18. Bidin, Z., Lojkic, I., Grce, M., Cajavec, S., and Pokric, B. (2001) Differentiation of infectious bursal disease virus strains at a genomic level. Veterinarski Arhiv 71 (6): 325-336.
19. Ikuta, N., El-Attrache, J., Villegas, P., Garcia, E.M., Lunge, V.R., Fonseca, A.S.K., Oliveira C. and Marques, E.K. (2001) Molecular characterization of Brazilian infectious bursal disease viruses. Avian Dis. 45: 297-306.
20. Owoade, A.A., Mulders, M.N., Kohnen, J., Ammerlaan, W., and Muller, C.P. (2004) High sequence diversity in infectious bursal disease virus serotype 1 in poultry and turkey suggest West African origin of very virulent strains. Arch. Virol, 149 (4): 653-72.
21. Adamu, J., Owoade, A.A., Abdu, P.A., Kazeem, H.M., and Fatihu, M.Y. (2013) Characterization of field and vaccine infectious bursal disease viruses from Nigeria revealing possible virulence and regional markers in the VP2 minor hydrophilic peaks. Avian Path, 42 (5): 420-33
22. Sellers, H.S., Villegas, P.N., Seal, B.S., and Jackwood, D.J. (1999) Antigenic and molecular characterization of three infectious bursal disease virus field isolates. Avian Dis. 43: 198-206.
23. Zierenberg, K., Raue, R., and Muller, H. (2001) Rapid identification of "very virulent" strains of Infectious bursal disease virus by reverse transcription-polymerase chain reaction combined with restriction enzyme analysis. Avian Path. 30: 55–62.
24. Kabell, S., Handberg, K.J., and Bisgaard, M. (2006) Impact of coccidial infection on vaccine- and vvIBDV in lymphoid tissues of SPF chickens as detected by RT-PCR. Acta Vet. Scand. 48:17.
25. Naqi, S., Thompson, G., Bauman, B., and Mohammed, H. (2001) The exacerbating effect of infectious bronchitis virus infection on the Infectious bursal disease virus induced suppression of opsonization of Escherichia coli antibody in chicken. Avian Dis, 45 (1):52-60.