Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test

Vet World Vol.11 April-2018 Article-11

Research Article

Veterinary World, 11(4): 474-479

https://doi.org/10.14202/vetworld.2018.474-479

K. Rathore¹, B. Joseph¹, D. K. Sharma^1,3, A. Gaurav², S. K. Sharma^1,3, M. Milind¹, P. Patel¹, C. Prakash⁴, and L. Singh⁵

1. Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.
2. Department of Veterinary Public Health, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.
3. Department of Veterinary Medicine, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.
4. Animal Health Division, CSWRI, Avikanagar, Malpura, Tonk, 304501 Rajasthan, India.
5. Department of Livestock Products Technology, College of Veterinary and Animal Science, Navania, Udaipur, 313601, Rajasthan, India.

Background and Aim: This study was designed to evaluate the potential of the use of multiplex polymerase chain reaction (PCR) as an alternative to conventional antibiotic sensitivity test.

Materials and Methods: Isolates of Staphylococcus aureus (total = 36) from clinical cases presented to Teaching Veterinary Clinical Complex of College of Veterinary and Animal Sciences (CVAS), Navania, Udaipur, were characterized by morphological, cultural, and biochemical methods. Then, the isolates were further subjected to molecular characterization by PCR targeting S. aureus-specific sequence (107 bp). Phenotypic antibiotic sensitivity pattern was analyzed by Kirby Bauer disc diffusion method against 11 commonly used antibiotics in veterinary medicine in and around Udaipur region. The genotypic antibiotic sensitivity pattern was studied against methicillin, aminoglycosides, and tetracycline targeting the gene mecA, aacA-aphD, and tetK by multiplex PCR.

Results: There was 100% correlation between the phenotype and genotype of aminoglycoside resistance, more than 90% correlation for methicillin resistance, and 58.3% in the case tetracycline resistance.

Conclusion: As there is a good correlation between phenotype and genotype of antibiotic resistance, multiplex PCR can be used as an alternative to the conventional antibiotic susceptibility testing, as it can give a rapid and true prediction of antibiotic sensitivity pattern. Keywords: antimicrobial resistance, genotype, phenotype, Staphylococcus aureus.

Keywords: antimicrobial resistance, genotype, phenotype, Staphylococcus aureus.

How to cite this article: Rathore K, Joseph B, Sharma DK, Gaurav A, Sharma SK, Milind M, Patel P, Prakash C, Singh L (2018) Evaluation of multiplex polymerase chain reaction as an alternative to conventional antibiotic sensitivity test, Veterinary World, 11(4): 474-479.

Received: 07-12-2017 Accepted: 12-03-2018 Published online: 13-04-2018

Corresponding author: B. Joseph E-mail: dr.binzy@gmail.com

DOI: 10.14202/vetworld.2018.474-479

Copyright: Rathore, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.