Molecular characterization of canine parvovirus variants (CPV-2a, CPV-2b, and CPV-2c) based on the VP2 gene in affected domestic dogs in Ecuador

Vet World Vol.11 April-2018 Article-12

Research Article

Veterinary World, 11(4): 480-487

https://doi.org/10.14202/vetworld.2018.480-487

David De la Torre^1,2, Eulalia Mafla¹, Byron Puga², Linda Erazo², Claudete Astolfi-Ferreira¹, and Antonio Piantino Ferreira¹

1. Department of Pathology, School of Veterinary Medicine, University of Sao Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270, Sao Paulo, Brazil.
2. Department of Pathology, School of Veterinary Medicine and Animal Science, Central University of Ecuador, EC170521, Quito, Ecuador.

Background and Aim: The objective of this study was to determine the presence of the variants of canine parvovirus (CPV)-2 in the city of Quito, Ecuador, due to the high domestic and street-type canine population, and to identify possible mutations at a genetic level that could be causing structural changes in the virus with a consequent influence on the immune response of the hosts.

Materials and Methods: Thirty-five stool samples from different puppies with characteristic signs of the disease and positives for CPV through immunochromatography kits were collected from different veterinarian clinics of the city. Polymerase chain reaction and DNA sequencing were used to determine the mutations in residue 426 of the VP2 gene, which determines the variants of CPV-2; in addition, four samples were chosen for complete sequencing of the VP2 gene to identify all possible mutations in the circulating strains in this region of the country.

Results: The results revealed the presence of the three variants of CPV-2 with a prevalence of 57.1% (20/35) for CPV-2a, 8.5% (3/35) for CPV-2b, and 34.3% (12/35) for CPV-2c. In addition, complete sequencing of the VP2 gene showed amino acid substitutions in residues 87, 101, 139, 219, 297, 300, 305, 322, 324, 375, 386, 426, 440, and 514 of the three Ecuadorian variants when compared with the original CPV-2 sequence.

Conclusion: This study describes the detection of CPV variants in the city of Quito, Ecuador. Variants of CPV-2 (2a, 2b, and 2c) have been reported in South America, and there are cases in Ecuador where CVP-2 is affecting even vaccinated puppies. Keywords: canine parvovirus, canine parvovirus-2, Ecuador, molecular characterization, variants.

Keywords: canine parvovirus, canine parvovirus-2, Ecuador, molecular characterization, variants.

How to cite this article: De la Torre D, Mafla E, Puga B, Erazo L, Astolfi-Ferreira C, Ferreira AP (2018) Molecular characterization of canine parvovirus variants (CPV-2a, CPV-2b, and CPV-2c) based on the VP2 gene in affected domestic dogs in Ecuador, Veterinary World, 11(4): 480-487.

Received: 02-02-2018 Accepted: 22-03-2018 Published online: 16-04-2018

Corresponding author: Antonio Piantino Ferreira E-mail: ajpferr@usp.br

DOI: 10.14202/vetworld.2018.480-487

Copyright: De la Torre, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.