Molecular detection of serotype groups of Listeria monocytogenes isolated from gallbladder of cattle and sheep in Iraq

Vet World Vol.11 April-2018 Article-4

Research Article

Veterinary World, 11(4): 431-436

https://doi.org/10.14202/vetworld.2018.431-436

Hamza Jawad Al-Ali¹, Mohsen Abd Al-Rodhan², Samer Abdulsahib Al-Hilali³, Alaa Hani Al-Charrakh⁴, Ali Muhsin Al-Mohana³, and Zainab Jaber Hadi³

1. Najaf Veterinary Hospital, Najaf Province, Iraq.
2. Department of Clinical and Laboratory Science, College of Pharmacy, University of Al-Qadisiyah , Diwaniyah, Iraq.
3. Department of Microbiology, College of Medicine, University of Kufa, Kufa, Iraq.
4. Department of Microbiology, College of Medicine, Babylon University, Hillah, Babylon Governorate, Iraq.

Background and Aim: This study was designed to investigate the occurrence of serotypes of Listeria monocytogenes, an important food-borne pathogen, in gallbladder samples from cattle and sheep.

Materials and Methods: Three hundred samples were collected and screened for the presence of L. monocytogenes. The identification of the isolates was confirmed by API-Listeria system and by the presence of hemolysin (hyl) gene. The isolates were subjected to polymerase chain reaction-based serotype identification with d1 (division 1), d2 (division 2), glt, mama (mismatch amplification mutation assay), and flaA (flagellin protein) genes.

Results: A total of 8 (2.7%) L. monocytogenes were recovered from 6 (4.0%) samples of sheep and 2 (1.3%) samples of cattle. All isolates showed positive results with Hly primers. Four isolates carried d1 gene, did not possess glt gene and harbored mama gene. The serotypes of these isolates were identified as 4a or 4c. The other 4 isolates carried d2 gene, 3 of them were positive with the FlaA primers, and hence, determined to be a 1/2a or 3a serotype, and 1 isolate was determined to be 1/2c or 3c serotype.

Conclusion: This study concluded that the presence of 1/2a serotype in gallbladder samples indicates public health risk through cross-contamination of meat at slaughterhouses. Keywords: cattle, gallbladder, Listeria monocytogenes, molecular detection, sheep.

Keywords: cattle, gallbladder, Listeria monocytogenes, molecular detection, sheep.

How to cite this article: Al-Ali HJ, Al-Rodhan MA, Al-Hilali SA, Al-Charrakh AH, Al-Mohana AM, Hadi ZJ (2018) Molecular detection of serotype groups of Listeria monocytogenes isolated from gallbladder of cattle and sheep in Iraq, Veterinary World, 11(4): 431-436.

Received: 03-11-2017 Accepted: 27-02-2018 Published online: 07-04-2018

Corresponding author: Alaa Hani Al-Charrakh E-mail: ahani67@gmail.com

DOI: 10.14202/vetworld.2018.431-436

Copyright: Al-Ali, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.