Angiogenesis activity of Jatropha curcas L. latex in cream formulation on wound healing in mice

Vet World Vol.11 July-2018 Article-9

Research Article

Veterinary World, 11(7): 939-943

https://doi.org/10.14202/vetworld.2018.939-943

Ummu Balqis¹, Darmawi^2,3, Cut Dahlia Iskandar⁴, and Muhammad Nur Salim^1,5

1. Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
2. Laboratory of Microbiology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
3. Laboratory of Research, Faculty of Veterinary Medicine, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.
4. Laboratory of Histology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
5. Study Program of Mathematics and Applied Sciences, Syiah Kuala University, Banda Aceh 23111, Indonesia.

Background and Aim: The aim of this research was to determine the angiogenesis activity of Jatropha curcas latex in cream formulation on CD34 immune expression during wound healing phase in mice skin.

Materials and Methods: Amount of 36 2-month-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. All mice were divided into four groups, namely the base cream as control group (A), sulfadiazine 0.1% cream (B), Jatropha curcas latex cream 10% (C), and J. curcas latex cream 15% (D). All groups were treated entire surface of wound. All experiments were performed twice a day for 10 days. Experiments were terminated on days 3, 7, and 10, respectively. The wound healing was assayed in stained histological section in immunohistochemical of the wounds. The CD34 expression was investigated under a microscope.

Results: The results showed that the cream from 10% and 15% latex J. curcas revealed moderate immune reaction to CD34 on days 3 and 7 in wound healing of mice skin.

Conclusion: We concluded that the cream from 10% and 15% latex J. curcas has potential as angiogenesis activity in wound healing of mice skin. Keywords: angiogenesis, CD34, Jatropha curcas latex cream, wound healing.

Keywords: angiogenesis, CD34, Jatropha curcas latex cream, wound healing.

How to cite this article: Balqis U, Darmawi, Iskandar CD, Salim MN (2018) Angiogenesis activity of Jatropha curcas L. latex in cream formulation on wound healing in mice, Veterinary World, 11(7): 939-943.

Received: 26-02-2018 Accepted: 05-06-2018 Published online: 15-07-2018

Corresponding author: Muhammad Nur Salim E-mail: mnursalim@unsyiah.ac.id

DOI: 10.14202/vetworld.2018.939-943

Copyright: Balqis, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.