Vet World Vol.11 October-2018 Article-10
Research Article
Veterinary World, 11(10): 1423-1427
https://doi.org/10.14202/vetworld.2018.1423-1427
Detection, characterization, and antibiogram of extended-spectrum beta-lactamase Escherichia coli isolated from bovine milk samples in West Bengal, India
2. Department of Veterinary Public Health, F/VAS, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.
Background and Aim: The study was aimed to isolate and characterize extended-spectrum beta-lactamase (ESBL)-producing E. coli from milk samples collected from different parts of West Bengal, India, to assess the potential risk associated with the food.
Materials and Methods: Around 182 milk samples were collected from apparently healthy cows reared by organized dairy farms in West Bengal. E. coli was isolated from collected samples as per standard methods followed by serotyping. The detection of ESBL-producing E. coli was done both phenotypically and genotypically by detecting the presence of blaCTX-M gene. Antibiogram of the ESBL-positive isolates was done using common 12 antibiotics by disc diffusion method.
Results: A total of 22 (12.1%) samples were found to be positive for E. coli in this study. Different serotypes such as O11, O20, O22, O34, O35, O128, O149, and UT were isolated from the collected samples. 12 (54.5%) E. coli strains showed the capability of producing ESBL, both phenotypically and genotypically with the presence of blaCTX-M gene. Antibiogram of these ESBL-positive isolates revealed the drugs such as colistin (100%), levofloxacin (83.33%), and imipenem (66.67%) to be highly sensitive against this pathogen but drugs such as cefotaxime (100%), ceftazidime (91.67%), amoxicillin/clavulanic acid (83.33%), tetracycline (75.00%), and gentamicin (58.33%) to be very much resistant.
Conclusion: More than 50% of the E. coli strains prevalent in the bovine milk samples were positive for ESBL production and are resistant to most of the common antimicrobials which may be alarming for human health. Keywords: antibiogram, blaCTX-M, bovine milk, extended-spectrum beta-lactamase, Escherichia coli.
Keywords: antibiogram, blaCTX-M, bovine milk, extended-spectrum beta-lactamase, Escherichia coli.
How to cite this article: Batabyal K, Banerjee A, Pal S, Dey S, Joardar SN, Samanta I, Isore DP, Singh AD (2018) Detection, characterization, and antibiogram of extended-spectrum beta-lactamase Escherichia coli isolated from bovine milk samples in West Bengal, India, Veterinary World, 11(10): 1423-1427.
Received: 23-07-2018 Accepted: 07-09-2018 Published online: 16-10-2018
Corresponding author: Kunal Batabyal E-mail: drkb.micro@gmail.com
DOI: 10.14202/vetworld.2018.1423-1427
Copyright: Batabyal, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.