Effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine

Vet World Vol.12 August-2019 Article-18

Research Article

Veterinary World, 12(8): 1299-1303

https://doi.org/10.14202/vetworld.2019.1299-1303

Nidhi P. Raval^1,2, Tejas M. Shah¹, Linz-Buoy George², and Chaitanya G. Joshi³

1. Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.
2. Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, India.
3. Gujarat Biotechnology Research Centre, Block B and D, 6th Floor, MS Building, Sector - 11, Gandhinagar, Gujarat, India.

Background and Aim: Studies have shown that the pH of the vagina during the course of fertilization may influence the migration of X- and Y-bearing spermatozoa and thus leading to skewness in the sex of the offspring. Hence, this study was carried out to check the effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine (Bos indicus).

Materials and Methods: To check the effect of pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine, we used buffers of various pH ranging from 5.5 to 9.0 for swim-up procedure of sperm sample and collected upper and bottom fraction from the same buffer and checked the abundance of X- and Y-bearing spermatozoa by droplet digital polymerase chain reaction using X- and Y-chromosome-specific DNA probe.

Results: The abundance of X- and Y-bearing spermatozoa was not differed significantly in either of the fraction collected.

Conclusion: Thus, it appears to be unlikely that an immediate impact of pH on sperm can be a solitary impact on the sex of offspring in bovine. Keywords: droplet digital polymerase chain reaction, spermatozoa, swim-up.

Keywords: droplet digital polymerase chain reaction, spermatozoa, swim-up.

How to cite this article: Raval NP, Shah TM, George LB, Joshi CG (2019) Effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine, Veterinary World, 12(8): 1299-1303.

Received: 27-04-2019 Accepted: 05-07-2019 Published online: 23-08-2019

Corresponding author: Chaitanya G. Joshi E-mail: dir-gbrc@gujarat.gov.in

DOI: 10.14202/vetworld.2019.1299-1303

Copyright: Raval, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.