Hepatoprotective effect of crude polysaccharides extracted from Ganoderma lucidum against carbon tetrachloride-induced liver injury in mice

Vet World Vol.12 December-2019 Article-15

Research Article

Veterinary World, 12(12): 1987-1991

https://doi.org/10.14202/vetworld.2019.1987-1991

Raden Joko Kuncoroningrat Susilo¹, Dwi Winarni¹, Saikhu Akhmad Husen¹, Suhailah Hayaza¹, Hunsa Punnapayak^1,2, Sri Puji Astuti Wahyuningsih¹, Elma Sakinatus Sajidah¹, and Win Darmanto¹

1. Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
2. Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.

Background and Aim: Natural products are currently widely used as alternative treatments for liver disease. The study aimed to determine the hepatoprotective effect of crude polysaccharides extracted from Ganoderma lucidum against liver injury induced by carbon tetrachloride (CCl4).

Materials and Methods: Twenty-four male BALB/C mice were randomly divided into six groups. Serum and liver samples were taken on day 10 after G. lucidum administration. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were measured using enzyme-linked immunosorbent assays, and the histology of the liver was evaluated using light microscopy.

Results: G. lucidum extract significantly decreased the levels of ALT, AST, and MDA and significantly increased the levels of SOD and CAT. In the histological evaluation, the liver tissue of CCl4-treated mice exhibited hydropic degeneration, necrosis, and sinusoidal dilatation. G. lucidum extract administration improved this liver tissue histopathology.

Conclusion: Crude polysaccharides extracted from G. lucidum showed a hepatoprotective effect, regenerating damaged liver tissue. Keywords: carbon tetrachloride, crude polysaccharide extract, Ganoderma lucidum, hepatotoxicity.

Keywords: carbon tetrachloride, crude polysaccharide extract, Ganoderma lucidum, hepatotoxicity.

How to cite this article: Susilo RJK, Winarni D, Husen SA, Hayaza S, Punnapayak H, Wahyuningsih SPA, Sajidah ES, Darmanto W (2019) Hepatoprotective effect of crude polysaccharides extracted from Ganoderma lucidum against carbon tetrachloride-induced liver injury in mice, Veterinary World, 12(12):1987-1991.

Received: 01-08-2019 Accepted: 08-11-2019 Published online: 17-12-2019

Corresponding author: Win Darmanto E-mail: windarmanto@fst.unair.ac.id

DOI: 10.14202/vetworld.2019.1987-1991

Copyright: Susilo RJK, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.