Vet World Vol.12 February-2019 Article-16
Research Article
Veterinary World, 12(2): 288-294
https://doi.org/10.14202/vetworld.2019.288-294
Toxocara vitulorum cuticle glycoproteins in the diagnosis of calves' toxocariasis
2. Department of Chemistry, Faculty of Science, Menoufia University, Shebin-El Kom, Egypt.
Background and Aim: The current study was designed to isolate and characterize Toxocara vitulorum glycoprotein antigens and then to evaluate its potency in accurate diagnosis of toxocariasis.
Materials and Methods: T. vitulorum glycoprotein fractions were isolated using Con-A affinity chromatography. The fractions characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and immunoblot assay. Mass spectrometric analysis was used for identification of proposed structure of the N-acetylglucosamine (GlcNAc) fraction. Enzyme-linked immunosorbent assay (ELISA) was used to assess the diagnostic potential of the isolated fractions.
Results: Surface of T. vitulorum adult worm revealed two glycoprotein fractions rich in glucose (Glc) and GlcNAc. Three bands of molecular weight 212kDa, 107 kDa, and 93 kDa were detected in Glc fraction by SDS-PAGE. These bands were also detected in GlcNAc fraction with an additional band of 49 kDa. GlcNAc fraction showed more diagnostic potency of calves' toxocariasis; 79% than Glc fraction; 46.9% by indirect ELISA. The additional band of 49 kDa in GlcNAc fraction is probably responsible for its higher diagnostic potentials. Western blotting verified the immunoreactivity of the Glc and GlcNAc isolated fraction as they reacted with calves sera infected with toxocariasis. The proposed structure of GlcNAc fraction was Ser-Meth-Arg-O-methylated GlcNAc.
Conclusion: GlcNAc-rich fraction of T. vitulorum can be successfully utilized in the diagnosis of calves' toxocariasis. Keywords: affinity chromatography, calves toxocariasis, Con A, enzyme-linked immunosorbent assay, mass spectrometric analysis, N-acetylglucosamine.
Keywords: affinity chromatography, calves toxocariasis, Con A, enzyme-linked immunosorbent assay, mass spectrometric analysis, N-acetylglucosamine.
How to cite this article: El Shanawany EE, Hassan SE, Abdel-Rahman AAH, Abdel-Rahman EH (2019) Toxocara vitulorum cuticle glycoproteins in the diagnosis of calves' toxocariasis, Veterinary World, 12(2): 288-294.
Received: 13-11-2018 Accepted: 14-01-2019 Published online: 20-02-2019
Corresponding author: Eman E. El Shanawany E-mail: ee.elshanawany@hotmail.com
DOI: 10.14202/vetworld.2019.288-294
Copyright: El Shanawany, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.