Staphylococcus aureus isolated from selected dairies of Algeria: Prevalence and susceptibility to antibiotics

Vet World Vol.12 February-2019 Article-3

Research Article

Veterinary World, 12(2): 205-210

https://doi.org/10.14202/vetworld.2019.205-210

Asmaa Manel Matallah¹, Leila Bouayad¹, Sofiane Boudjellaba², Faiza Mebkhout¹, Taha Mossadak Hamdi¹, and Nadjia Ramdani-Bouguessa³

1. Laboratory of Food Hygiene and Quality Insurance System (HASAQ), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
2. Research Laboratory Management of Local Animal Resources (GRAAL), Higher National Veterinary School, Rue Issad Abbes, Oued Smar, Algiers 16000, Algeria.
3. Laboratory of Medical Biology, Mohamed Mahmoudi City, Number 209 Bis, Baraki, Algiers, Algeria.

Background and Aim: The objectives of this study were to assess the prevalence of Staphylococcus aureus in raw milk in Algerian dairies, to study the effect of seasons on the contamination of milk and the susceptibility of isolated strains to antibiotics, and to estimate the risk on the health consumer.

Materials and Methods: The ISO method 6888-1 (1) was used for Staphylococcus screening. Antimicrobial susceptibility to the 11most used antibiotics in veterinary medicine was assessed using the disk diffusion assay.

Results: The overall prevalence was 31.56% (95/301); 34.84% (85/244) from raw milk collectors cisterns (MCC), 22.73% (5/22) from mixing tank milk before pasteurization, and 14.29% (5/35) from pasteurized tank milk (p<0.05). A significant difference (p<0.001) of contamination on MCC was observed between dairies without season influence (p≥0.05). It was observed that 49.47% of S. aureus isolates were resistant to penicillin, 5.26% to tetracycline, 4.21% to erythromycin, 3.15% to neomycin, 2.10% to cefoxitin, 2.10% to clindamycin, and 1.05% to ofloxacin. No resistance was observed for vancomycin, gentamicin, chloramphenicol, and trimethoprim-sulfamethoxazole.

Conclusion: A high prevalence of S. aureus from MCC was observed without significant effect of season. The pasteurization does not ensure the elimination of bacteria in all samples. Half of the isolates were resistant to penicillin. These findings emphasize the importance of S. aureus control in Algerian milk industry at different levels to improve public health. Keywords: antibiotic susceptibility, pasteurized milk, raw milk, Staphylococcus aureus.

Keywords: antibiotic susceptibility, pasteurized milk, raw milk, Staphylococcus aureus.

How to cite this article: Matallah AM, Bouayad L, Boudjellaba S, Mebkhout F, Hamdi TM, Ramdani-Bouguessa N (2019) Staphylococcus aureus isolated form selected dairies of Algeria: Prevalence and susceptibility to antibiotics, Veterinary World, 12(2): 205-210.

Received: 19-09-2018 Accepted: 26-12-2018 Published online: 08-02-2019

Corresponding author: Asmaa Manel Matallah E-mail: matallahasma@yahoo.fr

DOI: 10.14202/vetworld.2019.205-210

Copyright: Matallah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.