Phylogenetic characterization of Salmonella enterica from pig production and humans in Thailand and Laos border provinces

Vet World Vol.12 January-2019 Article-11

Research Article

Veterinary World, 12(1): 79-84

https://doi.org/10.14202/vetworld.2019.79-84

Rangsiya Prathan¹, Asinamai Athliamai Bitrus¹, Nuananong Sinwat^1,2, Sunpetch Angkititrakul³, and Rungtip Chuanchuen¹

1. Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
2. Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Kamphaengsean campus, Nakhonpathom, 73140 Thailand.
3. Research Group for Prevention Technology in Livestock, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40000, Thailand.

Background and Aim: The genetic relationship among serotypes of Salmonella enterica from food animals, food of animal origin, and human is of interest as the data could provide an important clue for the source of human infection. This study aimed to determine the genetic relatedness of S. enterica from pig production and human in Thailand-Laos border provinces.

Materials and Methods: A total of 195 S. enterica serotypes isolated from pig and pork (n=178) and human (n=17) including four serotypes (Typhimurium, Rissen, Derby, and Stanley) were randomly selected to examine their genetic relatedness using highly conserved sequence of three genes (fim A, man B, and mdh).

Results: The results showed that 195 Salmonella isolates of four different serotypes were grouped into five different clusters, and members of the same Salmonella serotypes were found in the same cluster. Salmonella isolated from pig production and human in Thailand-Laos border provinces represented overlapping population and revealed a high degree of similarity, indicating close genetic relationship among the isolates.

Conclusion: The results support that the determination of Salmonella serotyping combined with analysis of phylogenetic tree can be used track the clonal evolution and genetic diversity of Salmonella serotypes in different host species. Keywords: Laos, pig, Salmonella, Thailand.

Keywords: Laos, pig, Salmonella, Thailand.

How to cite this article: Prathan R, Bitrus AA, Sinwat N, Angkititrakul S, Chuanchuen R (2019) Phylogenetic characterization of Salmonella enterica from pig production and humans in Thailand and Laos border provinces, Veterinary World, 12(1): 79-84.

Received: 26-09-2018 Accepted: 29-11-2018 Published online: 16-01-2019

Corresponding author: Rungtip Chuanchuen E-mail: chuanchuen.R@gmail.com

DOI: 10.14202/vetworld.2019.79-84

Copyright: Prathan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.