The occurrence of disinfectant and antibiotic-resistant genes in Escherichia coli isolated from chickens in Egypt

Vet World Vol.12 January-2019 Article-21

Research Article

Veterinary World, 12(1): 141-145

https://doi.org/10.14202/vetworld.2019.141-145

Waleed A. Ibrahim¹, Sherif A. Marouf², Ahmed M. Erfan¹, Soad A. Nasef¹, and Jakeen K. El Jakee²

1. Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, P.O. Box 264-Dokki, Giza 12618, Egypt.
2. Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.

Background and Aim: This work aimed to determine the occurrence of antibiotic and disinfectant resistance genes in Escherichia coli isolated from chickens in Egypt.

Materials and Methods: Organs (liver, lung, heart, yolk sac, and bone marrow) of 1500 chicken samples were collected from diseased chickens suffered from colibacillosis with PM findings as CRD, diarrhea and omphalitis from different governorates of Egypt as: Giza, EL-Bahira, Fayoum, El-Dakahlia, El-Ismalia, and El-Sharkia during 2015-2016. These samples were labeled and transported immediately on ice to the Reference laboratory for quality control on poultry production (RLQP). The samples were cultured onto MacConkey agar and Eosin Methylene Blue Agar. Isolation and identification of the E. coli were performed based on morphology, cultural, staining, and biochemical properties. Antimicrobial resistance test was carried out using disk diffusion method. The PCR employing tetA, qacED1 and qacA/B were carried out for detection of these genes in isolated E.coli.

Results: The prevalence of E. coli in chicken was 34%. Predominant serotypes of E. coli which serologically identified were O128, O111, O44, O158, and O2. Antibiotic susceptibility test of E. coli revealed that 100% of isolates were resistant to ampicillin, erythromycin, and sulfamethoxazole-trimethoprim, while 73.53% and 38.23% of them were sensitive for colistin sulfate and levofloxacin, respectively. Antibiotic resistance genes as tetA gene were tested for isolated E. coli and detected by incidence rate of 91.18%. qac resistance genes resembling as qacED1 and qacA/B genes were detected in isolated E. coli 70.6% and 14.7%, respectively.

Conclusion: E. coli isolated from chickens in Egypt was carried qac and antibiotic-resistant genes that affect the poultry industry. Keywords: antimicrobial resistance, chickens, Egypt, Escherichia coli, qac resistance genes.

Keywords: antimicrobial resistance, chickens, Egypt, Escherichia coli, qac resistance genes.

How to cite this article: Ibrahim WA, Marouf SA, Erfan AM, Nasef SA, El Jakee JK (2019) The occurrence of disinfectant and antibiotic-resistant genes in Escherichia coli isolated from chickens in Egypt, Veterinary World, 12(1): 141-145.

Received: 01-10-2018 Accepted: 10-12-2018 Published online: 25-01-2019

Corresponding author: Waleed A. Ibrahim E-mail: waleed.abdelfattah@yahoo.com

DOI: 10.14202/vetworld.2019.141-145

Copyright: Ibrahim, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.