Detection of invA gene of Salmonella from milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique

Vet World Vol.12 January-2019 Article-25

Research Article

Veterinary World, 12(1): 170-175

https://doi.org/10.14202/vetworld.2019.170-175

Sheila Marty Yanestria¹, Reina Puspita Rahmaniar², Freshinta Jellia Wibisono¹, and Mustofa Helmi Effendi³

1. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Wijaya Kusuma University, Surabaya, Indonesia.
2. Department of Microbiology, Faculty of Veterinary Medicine, Wijaya Kusuma University, Surabaya, Indonesia.
3. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.

Background and Aim: The study aimed to detect the invA gene in Salmonella isolated from milkfish in the Sidoarjo wet fish market.

Materials and Methods: A total of 84 samples were prepared in enrichment media and isolated on the surface of Salmonella Shigella Agar. Salmonella growth produces transparent colonies with blackish color in the middle due to H2S gas formation. Samples were identified as Salmonella based on macroscopic colony morphology. Presumptive Salmonella sp. was put on Bismuth Sulfite Agar media. Salmonella was determined based on the results of the biochemical test that has been carried out using Microbact identification kits from negative gram staining.

Results: The results of this study indicate that 32 of 84 samples (38.09%) were Salmonella bacteria. Furthermore, the invA gene detection was carried out using the polymerase chain reaction technique. Electrophoresis results showed four positive samples contained invA gene with a length of 284 bp.

Conclusion: Results in this study indicate that contamination of milkfish with Salmonella needs strict hygienic measures to prevent their transmission to human. Keywords: human health, invA gene, milkfish, polymerase chain reaction, Salmonella.

Keywords: human health, invA gene, milkfish, polymerase chain reaction, Salmonella.

How to cite this article: Yanestria SM, Rahmaniar RP, Wibisono FJ, Effendi MH (2019) Detection of invA gene of Salmonella from milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique, Veterinary World, 12(1): 170-175.

Received: 03-09-2018 Accepted: 07-12-2018 Published online: 29-01-2019

Corresponding author: Mustofa Helmi Effendi E-mail: mheffendi@yahoo.com

DOI: 10.14202/vetworld.2019.170-175

Copyright: Yanestria, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.