Vet World Vol.12 January-2019 Article-9
Research Article
Veterinary World, 12(1): 68-71
https://doi.org/10.14202/vetworld.2019.68-71
Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction
2. Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
3. Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
Background and Aim: This study was conducted to study the coagulase (coa) gene-based genetic diversity of Staphylococcus aureus, isolated from different samples of cattle from three different regions in East Java Province, Indonesia.
Materials and Methods: A total of 160 raw milk samples collected in East Java Province, Indonesia, were screened for the presence of S. aureus. The presumptive isolates were confirmed by coa test. The confirmed S. aureus isolates were subjected to coa gene polymerase chain reaction.
Results: Of 160 different samples, 20 (12.5%) isolates of S. aureus were confirmed by positive coa test. Of 20 S. aureus isolates, 19 (95%) isolates carried coa gene. Six different genotypes of coa gene, i.e., 440 bp, 510 bp, 547 bp, 680 bp, 740 bp, and 820 bp were obtained. One coa genotypes, 510 bp (10 isolates) were observed in polymorphism to be more prevalent than the others, and the genotype was present in at least one isolates from every region.
Conclusion: It can be concluded that coa gene is easily epidemiological tool for detection of variation strain from S. aureus. Keywords: coagulase gene, coagulase test, polymorphism, raw milk, Staphylococcus aureus.
Keywords: coagulase gene, coagulase test, polymorphism, raw milk, Staphylococcus aureus.
How to cite this article: Effendi MH, Hisyam MAM, Hastutiek P, Tyasningsih W (2019) Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction, Veterinary World, 12(1): 68-71.
Received: 11-09-2018 Accepted: 19-11-2018 Published online: 10-01-2019
Corresponding author: Mustofa Helmi Effendi E-mail: mheffendi@yahoo.com
DOI: 10.14202/vetworld.2019.68-71
Copyright: Effendi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.