Vet World Vol.12 July-2019 Article-14
Research Article
Veterinary World, 12(7): 1022-1027
https://doi.org/10.14202/vetworld.2019.1022-1027
Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India
2. ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh, India.
3. ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, India.
4. ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India.
Background and Aim: The present study was carried out to find out the causative agent of exanthematous skin lesions in sheep maintained by Southern Regional Research Centre, Mannavanur, Kodai hills, Tamil Nadu.
Materials and Methods: Polymerase chain reaction (PCR) with Orf virus (ORFV) B2L gene-specific primers was carried out by employing the total genomic DNA isolated from the scabs as the template. The ORFV isolates from Kodai hills were characterized by the use of bioinformatics tools.
Results: The amino acid identity of ORFV isolate 1 from Kodai hills is having 98.14%, 96.29%, and 83.59% identity with reference strains of ORFV, Pseudocowpox virus, and bovine papular stomatitis virus, respectively. Phylogenetic analysis revealed that ORFV isolates from Kodai hills clustered with the other ORFV isolates from different geographical areas of India.
Conclusion: The etiological agent of exanthematous skin lesion among sheep of Kodai hills is ORFV. Keywords: B2L gene, Kodai hills, Orf virus, phylogenetic analysis, sheep.
Keywords: B2L gene, Kodai hills, Orf virus, phylogenetic analysis, sheep.
How to cite this article: Nagarajan G, Pourouchottamane R, Reddy GBM, Yogisharadhya R, Sumana K, Rajapandi S, Murali G, Thirumaran SMK, Mallick PK, Rajendiran AS (2019) Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India, Veterinary World, 12(7):1022-1027.
Received: 07-02-2019 Accepted: 03-06-2019 Published online: 12-07-2019
Corresponding author: G. Nagarajan E-mail: gnagarajanars@gmail.com
DOI: 10.14202/vetworld.2019.1022-1027
Copyright: Nagarajan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.